Computer analysis of two-dimensional gels.

Computer analysis of two-dimensional gels.
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二维凝胶的计算机分析。

DOI:
10.1016/0003-2697(81)90291-8
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发表时间:
1981
影响因子:
2.9
通讯作者:
Xuong,NH
Xuong,NH
中科院分区:
生物学4区
文献类型:
--
作者:
Vo,KP;Miller,MJ;Geiduschek,EP;Nielsen,C;Olson,A;Xuong,NH

文献摘要

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本文介绍了新的方法和计算机程序,使人们能够分析二维凝胶电泳产生的放射自显影图。这些程序完全自动地找到由这种凝胶分辨的斑点并定量其中的放射性。半自动程序也被开发出来,以匹配不同放射自显影图的斑点模式,并通过一系列凝胶跟踪任何单个多肽的合成。
New methods and computer programs are described which enable one to analyze autoradiograms produced by two-dimensional gel electrophoresis. These programs are completely automatic with respect to finding spots resolved by such gels and quantitating the radioactivity in them. Semiautomatic programs have also been developed to match the spot patterns of different autoradiograms, and to follow the synthesis of any individual polypeptide through a series of gels.