Identification and biochemical characterization of five long-chain acyl-coenzyme A synthetases from the diatom Phaeodactylum tricornutum
Identification and biochemical characterization of five long-chain acyl-coenzyme A synthetases from the diatom Phaeodactylum tricornutum
复制标题
三角褐指藻 5 种长链酰基辅酶 A 合成酶的鉴定及生化表征
DOI：
10.1016/j.plaphy.2013.10.036
复制
发表时间：
2014-01-01
影响因子：
6.5
通讯作者：
Gong, Yangmin
中科院分区：
文献类型：
--
作者：
Guo, Xiaojing;Jiang, Mulan;Gong, Yangmin
长链酰基辅酶A合成酶（ACSL；EC 6.2.1.3）催化游离脂肪酸转化为酰基辅酶A酯，这对于脂肪酸和脂质代谢的许多途径是必需的。硅藻三角褐指藻基因组编码五个假定的 ACSL (PtACSL1-5)，它们包含几个高度保守的基序，并且彼此之间以及与其他已知 ACSL 之间具有有限的序列相似性。为了验证它们的长链酰基辅酶A合成酶活性，克隆、表达了编码这些PtACSL的五种cDNA，并测试了它们补充酿酒酵母双突变体FAA1 Delta FAA4 Delta的能力。五个 PtACSL 中只有两个能够恢复生长、促进外源脂肪酸摄取并增强储存脂质积累。我们还发现三角褐藻细胞能够从细胞外环境输入长链脂肪酸。三角褐藻ACSL的鉴定将为ACSL介导的硅藻脂质合成和代谢的研究提供分子基础。 (C) 2013 Elsevier Masson SAS。版权所有。
Long-chain acyl-CoA synthetase (ACSL; EC 6.2.1.3) catalyzes the conversion of free fatty acid to acyl-CoA ester, which is necessary for many pathways of fatty acid and lipid metabolism. The diatom Phaeodactylum tricornutum genome encodes five putative ACSLs (PtACSL1-5) that contain several highly conserved motifs and share limited sequence similarities with each other and with other known ACSLs. To verify their long-chain acyl-CoA synthetase activities, five cDNAs encoding these PtACSLs were cloned, expressed, and tested for their ability to complement the Saccharomyces cerevisiae double mutant FAA1 Delta FAA4 Delta. Only two of five PtACSLs were able to restore growth, facilitate exogenous fatty acid uptake, and enhance storage lipid accumulation. We also found that P. tricornutum cells are capable of importing long-chain fatty acids from extracellular environment. The identification of P. tricornutum ACSLs will provide molecular basis for the study of ACSL-mediated lipid synthesis and metabolism in diatoms. (C) 2013 Elsevier Masson SAS. All rights reserved.