The vascular protective effects of Anoectochilus roxburghii polysaccharose under high glucose conditions
The vascular protective effects of Anoectochilus roxburghii polysaccharose under high glucose conditions
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金线莲多糖的血管保护作用
DOI：
10.1016/j.jep.2017.03.012
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发表时间：
2017-04-18
影响因子：
5.4
通讯作者：
Li, Ya
中科院分区：
文献类型：
--
作者：
Liu, Zhen-ling;Zhang, Jian-Gang;Li, Ya
民族药理意义：金线莲在中国作为保健食品和治疗糖尿病的草药已有数百年的历史。金线莲多糖(ARP)是金线莲的主要活性成分。目的：研究金线莲多糖在体内和体外的血管保护作用。材料和方法：检测ARP对糖尿病小鼠的降血糖作用。结果：与未治疗的糖尿病小鼠相比，ARP(100和300 mg/kg)能显著降低糖尿病小鼠的血糖水平。组织学检查显示，ARP对血管内皮细胞的损伤有不同程度的改善，尤以300 mg/kg剂量的ARP更为明显。体外实验表明，ARP(10、20、30 mU/m L)可显著抑制HG诱导的HUVEC产生的活性氧(ROS)、单核细胞趋化蛋白-1(MCP-1)和细胞间黏附分子-1(ICAM-1)。ARP预处理不仅通过增加基质金属蛋白酶组织抑制因子(TIMPs)的表达来抑制HG诱导的基质金属蛋白酶(MMPs)活性，而且通过调节MMPs/TIMPs的平衡来维持血管结构的动态平衡。ARP可显著降低HG诱导的HUVEC中p-NF-kappa B p65、p-p38 MAPK的表达水平。结论：ARP的血管保护作用可能与NF-kappa B和p38 MAPK信号通路有关。ARP可作为治疗糖尿病血管病变的有用物质。
Ethnopharmacological relevance: Anoectochilus roxburghii has been used as a health food and a herb for treatment diabetes in China for hundreds years. Anoectochilus roxburghii polysaccharose (ARP) is the major active component of the plant.Aim of the study: The present study investigated the vascular protection of ARP in vivo and in vitro experiments.Materials and methods: Hypoglycemic activity of ARP was examined in diabetic mice. Moreover, the further vascular protective effects in vitro were investigated in human umbilical vein endothelial cells (HUVECs) stimulated by high glucose (HG, 35 mM).Results: Compared with untreated diabetic mice, ARP (100 or 300 mg/kg) caused a significant decrease in blood glucose levels. Histological examination showed that ARP ameliorated endothelial damage to some extent, especially ARP at dosage of 300 mg/kg. In vitro assay, pretreatment with ARP (10, 20 and 30 mu g/mL) markedly inhibited generations of reactive oxygen species (ROS), monocyte chemoattractant protein-1 (MCP-1) and intercellular adhesion molecule-1 (ICAM-1) in HG-induced HUVECs. ARP pretreatment not only suppressed HG-induced matrix metalloproteinases (MMPs) activity via increasing the expression of the tissue inhibitors of MMPs (TIMPs), but also adjusted the MMPs/TIMPs balance to maintain homeostasis of vascular structure. Moreover, pretreatment with ARP could significantly reduce p-NF-kappa B p65, p-p38 MAPK expression levels in HG-induced HUVECs.Conclusions: The vascular protective effects of ARP might be associated with NF-kappa B and p38 MAPK pathway. ARP might be used as useful substance in the treatment of vasculopathy in diabetic patients.