A 14;18 and an 8;14 chromosome translocation in a cell line derived from an acute B-cell leukemia.
A 14;18 and an 8;14 chromosome translocation in a cell line derived from an acute B-cell leukemia.
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急性 B 细胞白血病细胞系中的 14;18 和 8;14 染色体易位。
DOI：
10.1073/pnas.81.22.7166
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发表时间：
1984
影响因子：
11.1
通讯作者：
Croce,CM
中科院分区：
文献类型：
--
作者：
Pegoraro,L;Palumbo,A;Erikson,J;Falda,M;Giovanazzo,B;Emanuel,BS;Rovera,G;Nowell,PC;Croce,CM
我们已经建立了一个细胞系，我们命名为380，从一个年轻的男性急性淋巴细胞白血病（FAB型L2）。该细胞系的核型分析表明，它携带8;14和14;18染色体易位，这是伯基特淋巴瘤和滤泡性淋巴瘤的特征，分别。该细胞系为EB病毒抗原阴性，与B细胞特异性单克隆抗体反应，含有重排的免疫球蛋白重链和轻链基因，但不表达人免疫球蛋白。在该细胞系中，两个mu重链恒定（C mu）基因座在连接（JH）DNA片段内重排。其中一条14 q+染色体上的一个JH片段与8号染色体的一个片段重排，其中c-myc癌基因位于该片段，而另一个与18号染色体的一个片段重排，其中一个假定的癌基因，我们称之为bcl-2，位于该片段。c-myc癌基因易位到14 q+染色体之一，在其种系构型中，距离JH区段和位于JH和mu开关区之间的重链增强子超过14个碱基。基于这些研究结果，我们提出了一个模型的某些方面的B细胞肿瘤的发生，根据该B细胞肿瘤进行易位涉及重链基因座上的两个人类染色体14是一个多步骤的过程的结果。
We have established a cell line, which we named 380, from a young male with acute lymphoblastic leukemia (FAB type L2). Karyologic analysis of this cell line indicates that it carries an 8;14 and a 14;18 chromosome translocation, which are characteristic of Burkitt lymphoma and of follicular lymphoma, respectively. This cell line is Epstein-Barr virus antigen-negative, reacts with monoclonal antibodies specific for B cells, and contains rearranged immunoglobulin heavy and light chain genes, but does not express human immunoglobulins. In this cell line, both mu heavy chain constant (C mu) loci are rearranged within the joining (JH) DNA segment. One of the JH segments on one of the 14q+ chromosomes is rearranged with a segment of chromosome 8, where the c-myc oncogene resides, while the other is rearranged with a segment of chromosome 18 where a putative oncogene, which we have called bcl-2, is located. The c-myc oncogene, which is translocated to one of the 14q+ chromosomes, is in its germ-line configuration more than 14 kilobases away from both the JH segment and the heavy chain enhancer that is located between the JH and mu switch region. Based on these findings, we propose a model of some aspects of B-cell oncogenesis according to which B-cell neoplasms carrying translocations involving the heavy chain loci on both human chromosomes 14 are the result of a multiple step process.