Ginsenoside-Rd potentiates apoptosis induced by hydrogen peroxide in basilar artery smooth muscle cells through the mitochondrial pathway
Ginsenoside-Rd potentiates apoptosis induced by hydrogen peroxide in basilar artery smooth muscle cells through the mitochondrial pathway
复制标题
人参皂苷-Rd 通过线粒体途径增强过氧化氢诱导的基底动脉平滑肌细胞凋亡
DOI：
10.1007/s10495-011-0671-4
复制
发表时间：
2012-02-01
期刊：
影响因子：
7.2
通讯作者：
Guan, Yong-Yuan
中科院分区：
文献类型：
--
作者：
Li, Shi-Yang;Wang, Xiao-Guang;Guan, Yong-Yuan
我们前期的研究表明，三七皂苷-Rd具有抑制细胞增殖和逆转基底动脉重塑的作用。本研究的目的是探讨抗氧化剂- Rd是否影响过氧化氢诱导的基底动脉平滑肌细胞（BASMCs）凋亡。结果表明，黄芪皂苷-Rd能显著增强H2 O2诱导的细胞死亡和凋亡。这导致细胞活力的浓度依赖性降低。人参皂甙Rd可进一步增加细胞色素C释放和caspase-9/caspase-3活性，降低线粒体膜电位（MMP）稳定性和Bcl-2/Bax比值。细胞膜通透性转换抑制剂环孢霉素A（Cyclosporine A）可抑制H2 O2引起的线粒体通透性改变，并逆转黄芪皂苷Rd对MMP的影响。我们的数据有力地表明，三七皂苷-Rd增强H2 O2诱导的BASMCs的凋亡，通过依赖的途径。
Our previous studies showed that ginsenoside-Rd, a purified component from Panax notoginseng, inhibited cell proliferation and reversed basilar artery remodeling. The aim of this study was to investigate whether ginsenoside- Rd influences H2O2-induced apoptosis in basilar artery smooth muscle cells (BASMCs). The results showed that ginsenoside-Rd significantly potentiated H2O2-induced cell death and cell apoptosis. This resulted in a concentration-dependent reduction of the cell viability. Ginsenoside-Rd further increased cytochrome C release and caspase-9/caspase-3 activations, and reduced the stability of mitochondrial membrane potential (MMP) and the ratio of Bcl-2/Bax. Cyclosporine A, an inhibitor of mitochondrial-permeability transition, inhibited alteration of mitochondrial permeability induced by H2O2and reversed the effect of ginsenoside-Rd on MMP. Our data strongly suggest that ginsenoside-Rd potentiated H2O2-induced apoptosis of BASMCs through the mitochondria-dependent pathway.