p,p '-DDE Induces Gonadal Intersex in Japanese Medaka (Oryzias latipes) at Environmentally Relevant Concentrations: Comparison with o,p '-DDT
p,p '-DDE Induces Gonadal Intersex in Japanese Medaka (Oryzias latipes) at Environmentally Relevant Concentrations: Comparison with o,p '-DDT
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p,p '-DDE 在环境相关浓度下诱导日本青鳉 (Oryzias latipes) 的性腺双性：与 o,p '-DDT 的比较
DOI：
10.1021/acs.est.5b05042
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发表时间：
2016
影响因子：
11.4
通讯作者：
Hu JY
中科院分区：
文献类型：
--
作者：
Sun Jianxian;Wang Chen;Peng Hui;Zheng Guomao;Zhang Shiyi;Hu Jianying;Hu JY
以前的研究报告说，在世界各地的野生鱼类中，二氯二苯三氯乙烷（DDT）及其代谢物的身体负担很高。本研究通过对转基因日本稻（Oryzias latipes）孵育100天，评价了1,1-二氯-2,2-双（对氯苯基）-乙烯（p,p′-DDE）和do，p′-DDT对日本稻（Oryzias latipes）性腺发育和生殖的不良影响。p′-DDE和p′-DDT均可诱导雄鳉鱼发生双性行为，但p′-DDT的最低观察有效浓度（LOEC）为57.7 ng/g ww，比p′-DDE （272 ng/g ww）低约5倍。由于这两种化学品的loec与野生鱼类体内的浓度相当，因此滴滴涕污染可能会导致在野生鱼类中观察到的双性现象的发生。暴露于p′-DDT后，雄性肝脏中卵黄原蛋白的表达量明显高于p′-DDE，这与p′-DDT诱导雌雄间性的效果较p′-DDE强一致。这一现象可以部分解释为，除了通过雌激素受体产生的雌激素活性外，暴露于ddt的男性血浆中的17β-雌二醇水平也显著升高。13只双性雄虫受精率（p= 0.006）和孵化率（p= 0.019）均显著降低。本研究首次证实了环境相关浓度的p，p ' -DDE和do，p ' -DDT诱导双性和生殖效应。
Previous studies have reported high body burdens of dichlorodiphenyltrichloroethane (DDT) and its metabolites in wild fishes worldwide. This study evaluated the adverse effects of 1,1-dichloro-2,2-bis (p-chlorophenyl)-ethylene (p,p′-DDE) ando,p′-DDT on gonadal development and reproduction by exposing transgenic Japanese medaka (Oryzias latipes) from hatch for 100 days. While bothp,p′-DDE ando,p′-DDT induced intersex in male medaka, the lowest observable effective concentration (LOEC) ofo,p′-DDT was 57.7 ng/g ww, about 5-fold lower than that (272 ng/g ww) ofp,p′-DDE. Since LOECs of both chemicals were comparable to the body concentrations in wild fish, DDT contamination would likely contribute to the occurrence of intersex observed in wild fish. Exposure too,p′-DDT resulted in much higher expression of vitellogenin in liver of males thanp,p′-DDE, accordant with the higher potency ofo,p′-DDT thanp,p′-DDE to induce intersex. This phenomenon could be partly explained by the significantly elevated levels of 17β-estradiol in plasma of males exposed too,p′-DDT, in addition to its estrogenic activity via the estrogen receptor. Significantly lower fertilization (p= 0.006) and hatchability (p= 0.019) were observed in the 13 intersex males. This study for the first time demonstrated the induction of intersex and reproductive effects ofp,p′-DDE ando,p′-DDT at environmentally relevant concentrations.