Influence of epithelial-mesenchymal interaction on the viability of facial mesenchyme. II: Synthesis of basement-membrane components during tissue recombination.
Influence of epithelial-mesenchymal interaction on the viability of facial mesenchyme. II: Synthesis of basement-membrane components during tissue recombination.
复制标题
上皮间质相互作用对面部间质活力的影响。
DOI：
10.1002/ar.1092280110
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Minkoff,R
中科院分区：
文献类型：
--
作者：
Xu,ZL;Parker,SB;Minkoff,R
使用抗层粘连蛋白和IV型胶原的单抗确定组织分离过程中基底膜成分的存在。此外，在分离的上皮和间质以及组织重组中观察了基底膜成分的重建和基底膜的形成。鸡胚胎上颌突的上皮和间质被各种方案分离，包括先前研究中采用的方案(Saber等人：Anat。录制。225：56-66,1989)。结果表明，先前采用的方法在酶组织分离后不能去除基底膜成分。在重组前从分离的组织中去除基底膜成分(即层粘连蛋白和IV型胶原)的修订方案显示，细胞活力的发育间隔和梯度在大小和维度上与Saber等人的研究中观察到的相似(同上)。存在于重组外植体的间质中。因此，在组织重组过程中，IV型胶原和层粘连蛋白在最初似乎并不是必需的，以便表达随后的生长维持效应。然而，更多的研究表明，基底膜成分的合成不仅发生在单独的组织中，而且由于组织重组而显著改变。分离组织培养24小时可诱导上皮层粘连蛋白合成，24小时可诱导间充质胶原合成。然而，上皮和间充质的重组导致在1小时内快速诱导层粘连蛋白的合成。24小时后，上皮与间充质的重组导致层粘连蛋白不仅存在于上皮中，而且也存在于间充质中。这两种组织都是形成基底膜所必需的，在培养24小时后，基底膜似乎完全重建。这些观察表明，培养中的重组改变了这些基底膜成分的合成活性模式。在最近的一项研究中(Saber等人，19891)，利用器官培养中的各种组织构型进行了分离和重组实验，以确定上颌突上皮在面部原基发育过程中对间充质细胞活性的影响程度。本研究表明，早期胚胎面部间充质细胞从上皮细胞中分离出来并在器官培养中生长时，其活力受到严重损害。此外，还发现上皮细胞对间充质细胞活力的影响具有阶段依赖性。在发育年龄较大的人中，对维持生存能力的上皮细胞的要求不那么明显。它
The presence of basement-membrane components during tissue separation procedures was determined employing monoclonal antibodies to laminin and type IV collagen. In addition, the reconstitution of basement-membrane components and the formation of the basement-membrane were examined in isolated epithelium and mesenchyme and in tissue recombination. Epithelium and mesenchyme of maxillary processes of chick embryos were separated by a variety of protocols, including those employed in a prior study (Saber et al: Anat. Rec. 225: 56-66, 1989). Results indicated that the protocol previously employed did not remove basement-membrane components after enzymatic tissue separation. A revised protocol in which the basement-membrane components (ie, laminin and type IV collagen) were removed from isolated tissues prior to recombination revealed that a developmental compartment and a gradient of cell viability, comparable in size and dimensions to that observed in the study of Saber et al.(ibid.) was present in the mesenchyme of recombined explants. Type IV collagen and laminin, therefore, do not appear to be required initially during tissue recombination in order for subsequent growth-sustaining effects to be expressed. Additional studies revealed, however, that synthesis of basement-membrane components occurred not only in isolated tissues but was altered markedly by tissue recombination. Culture of isolated tissues demonstrated induction of laminin synthesis in separated epithelium by 24 hours and induction of collagen synthesis in isolated mesenchyme by 24 hours. Recombination of epithelium and mesenchyme, however, resulted in rapid induction of laminin synthesis within 1 hour. Recombination of epithelium and mesenchyme after 24 hours resulted in the presence of laminin not only in epithelium but in mesenchyme as well. Both tissues were required for basementmembrane formation which appeared to be fully reconstituted by 24 hours in culture. These observations indicate that recombination in culture alters the pattern of synthetic activity of these basement-membrane components. These can be characterized as “early”(temporal) and “late”(spatial) responses by the recombined tissues.In a recent study (Saber et al., 19891, separation and recombination experiments were performed employing a variety of tissue configurations in organ culture in order to determine the extent to which the epithelium of the maxillary process influences the viability of mesenchyme during the development of the facial primordia. This study indicated that the viability of facial mesenchyme of early stage embryos was severely impaired when separated from overlying epithelium and grown in organ culture. In addition, it was found that the influence of epithelium on the viability of mesenchyme was stage dependent. The requirement for the presence of epithelium for the maintenance of viability was less pronounced at older developmental ages. It