Analysis of the Genome Sequence and Prediction of B-Cell Epitopes of the Envelope Protein of Middle East Respiratory Syndrome-Coronavirus.
Analysis of the Genome Sequence and Prediction of B-Cell Epitopes of the Envelope Protein of Middle East Respiratory Syndrome-Coronavirus.
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中东呼吸综合征冠状病毒包膜蛋白基因组序列分析及B细胞表位预测
DOI：
10.1109/tcbb.2017.2702588
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发表时间：
2018-07
期刊：
影响因子：
--
通讯作者：
Zhao W
中科院分区：
文献类型：
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作者：
Xie Q;He X;Yang F;Liu X;Li Y;Liu Y;Yang Z;Yu J;Zhang B;Zhao W
2015年4月在韩国爆发的中东呼吸综合征冠状病毒（MERS-CoV）导致186人感染，截至2015年10月底，37人死亡。MERS-CoV是从中国输入的患者中分离的。MERS-CoV的包膜蛋白（E）是MERS-CoV的一个小的结构蛋白，在宿主识别和感染过程中起着重要作用。为了鉴定E蛋白的保守表位，通过比较来自过去引发严重大流行和感染人类的42种MERS-CoV毒株的E蛋白进行序列分析。为了预测E蛋白的潜在B细胞表位，使用三种最有效的在线表位预测程序，来自LaserGene软件的ABCpred、Bepipred和Protean程序。所有的核苷酸和氨基酸序列均获自NCBI数据库。一个具有合适长度（氨基酸58-82）的潜在表位被证实并预测为高度抗原性的。该表位在ABCpred程序中得分>0.80，在Bepipred程序中得分为0.35。由于PDB数据库中缺乏E蛋白的X射线晶体结构，因此还使用PHYRE 2和Pymol程序预测了E蛋白的模拟三维结构。总之，利用生物信息学方法，我们分析了MERS-CoV的基因组序列，并确定了E蛋白的潜在B细胞表位，这可能会显着改善我们目前的MERS疫苗开发策略。
The outbreak of Middle East respiratory syndrome-coronavirus (MERS-CoV) in South Korea in April 2015 led to 186 infections and 37 deaths by the end of October 2015. MERS-CoV was isolated from the imported patient in China. The envelope (E) protein, a small structural protein of MERS-CoV, plays an important role in host recognition and infection. To identify the conserved epitopes of the E protein, sequence analysis was performed by comparing the E proteins from 42 MERS-CoV strains that triggered severe pandemics and infected humans in the past. To predict the potential B cell epitopes of E protein, three most effective online epitope prediction programs, the ABCpred, Bepipred, and Protean programs from the LaserGene software were used. All the nucleotides and amino acids sequences were obtained from the NCBI Database. One potential epitope with a suitable length (amino acids 58–82) was confirmed and predicted to be highly antigenic. This epitope had scores of >0.80 in ABCpred and level 0.35 in Bepipred programs. Due to the lack of X-ray crystal structure of the E protein in the PDB database, the simulated 3D structure of the E protein were also predicted using PHYRE 2 and Pymol programs. In conclusion, using bioinformatics methods, we analyzed the genome sequence of MERS-CoV and identified a potential B-cell epitope of the E protein, which might significantly improve our current MERS vaccine development strategies.