Comparative transcriptome analysis of Discosporangium mesarthrocarpum (Phaeophyceae), Shizocladia ischiensis (Schizocladiophyceae), and Phaeothamnion confervicola (Phaeothamniophyceae), with special reference to cell wall-related genes
Comparative transcriptome analysis of Discosporangium mesarthrocarpum (Phaeophyceae), Shizocladia ischiensis (Schizocladiophyceae), and Phaeothamnion confervicola (Phaeothamniophyceae), with special reference to cell wall-related genes
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Discosporangium mesarthrocarpum（Phaeophyceae）、Shizocladia ischiensis（Schizocladiophyceae）和 Phaeothamnion confervicola（Phaeothamniophyceae）的比较转录组分析，特别涉及细胞壁相关基因
DOI：
10.1111/jpy.12190
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发表时间：
2014
影响因子：
2.9
通讯作者：
H
中科院分区：
文献类型：
--
作者：
Yamagishi;T.;Müller;D. G.;Kawai;H
在层藻谱系中，只有褐藻（褐藻纲）进化出复杂的多细胞性，尽管该谱系的一些其他成员（例如，裂枝藻属（Schizocladiophyceae）;棕枝藻属（Phaeotherophyceae）也发育简单的多细胞菌体。粘附性细胞外基质（ECM）的发育被认为是多细胞生物进化的关键步骤之一，因为ECM参与细胞相互粘附以及发育、生殖和复杂防御系统所必需的细胞间通讯。由于褐藻中不存在单细胞生物，我们认为与其他与褐藻密切相关的具有多细胞菌体的层藻类群的比较可以为阐明褐藻中多细胞生物的进化提供线索。本研究对三种具丝藻的转录组进行了研究，其中中节盘孢囊菌（Discosporangium mesarthrocarpum）被认为是褐藻族（Phaeophyceae）、伊斯基盘孢囊菌（S. ischiensis）和密生盘孢囊菌（P. confervicola）中最基本的类群之一。我们使用454-FLX高通量焦磷酸测序来生成这些物种的表达序列标签（EST）数据库，并在这些数据库与棕色藻类硅果海桐（Ectocarpus siliculosus）的基因组序列之间进行比较分析。结果表明，中节节圆藻细胞壁多糖代谢途径与硅土艾美耳球虫相似，而伊氏圆藻和密生圆藻细胞壁多糖代谢途径弗罗姆硅土艾美耳球虫明显不同，表明伊氏圆藻和密生圆藻细胞壁的组成可能与硅土艾美耳球虫不同。
Within the stramenopile lineage, only brown algae (Phaeophyceae) have evolved complex multicellularity, although some other members of the lineage (e.g.,Schizocladiain Schizocladiophyceae;Phaeothamnionin Phaeothamniophyceae) also develop simple multicellular thalli. The development of an adherent extracellular matrix (ECM) is considered to be one of the key steps in the evolution of multicellularity, because ECM is involved in adhesion of cells to each other and in cell–cell communication essential for developmental, reproductive, and sophisticated defense systems. Because there are no unicellular organisms within brown algae, we considered that comparison of other stramenopile taxa closely related to brown algae and having multicellular thalli could yield clues to elucidate the evolution of multicellularity in brown algae. In this study, we investigated transcriptomes involved in cell wall polysaccharide metabolism of three stramenopile species,Discosporangium mesarthrocarpum, which is suggested to be one of the most basal taxa within Phaeophyceae,S. ischiensis, andP. confervicola. We employed 454‐FLX high‐throughput pyrosequencing to generate expressed sequence tag (EST) databases for these species, and performed comparative analyses between these databases and the genome sequence of the brown algaEctocarpus siliculosus. Results indicate that cell wall polysaccharide metabolism pathways ofD. mesarthrocarpumare similar toE. siliculosus, whereas those ofS. ischiensisandP. confervicolaare significantly different fromE. siliculosus, suggesting that the components of the cell wall inS. ischiensisandP. confervicolaare likely to be different from those ofE. siliculosus.