Optic Nerve Crush Mice Followed Longitudinally with Spectral Domain Optical Coherence Tomography
Optic Nerve Crush Mice Followed Longitudinally with Spectral Domain Optical Coherence Tomography
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DOI：
10.1167/iovs.10-6311
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发表时间：
2011-04-01
影响因子：
4.4
通讯作者：
Wollstein, Gadi
中科院分区：
文献类型：
--
作者：
Gabriele, Michelle L.;Ishikawa, Hiroshi;Wollstein, Gadi
目的。应用光谱域光学相干断层扫描(SD-OCT)技术测量小鼠视网膜厚度，探讨视神经挤压伤的纵向效应。方法：C57BL/6小鼠单眼视神经在视神经直视下夹伤3s，距眼球后1 mm。手术前用SD-OCT(1.5×1.5×2.0 mm)扫描视神经头(ONH)，伤后重复扫描最多32d。一组没有接触神经挤压程序的小鼠作为对照。En Face SD-OCT图像用于手动将后续扫描与基线En Face图像对齐。每个随访日的总视网膜厚度(TRT)(沿着以ONH为中心的半径为0.33-0.42 mm的采样带)使用定制软件自动量化以进行全球和部门测量。结果：11只神经挤压小鼠(基线年龄76+/-11.8d)的11只眼和4只健康小鼠(基线年龄+/-0d)的8只眼被纳入研究对象。对照眼在整个随访过程中表现出轻微、渐进和持续的TRT增加。神经损伤眼表现出最初的增厚，随后变薄，第21天后略有反弹。早期增厚消退后观察到的厚度下降与对照眼有统计学差异(P<；0.05用于全局和部分测量)。结论SD-OCT可用于定量监测小鼠视网膜厚度随时间的变化。(投资眼科VS科学。2011年；52：2250-2254)doi：10.1167/iovs.106311
PURPOSE. To investigate the longitudinal effect of optic nerve crush injury in mice by measuring retinal thickness with spectral-domain optical coherence tomography (SD-OCT).METHODS. Optic nerves of one eye from each C57Bl/6 mouse were crushed under direct visualization for 3 seconds, 1 mm posterior to the globe. The optic nerve head (ONH) was imaged with SD-OCT (1.5 x 1.5 x 2.0 mm scan) before the surgical intervention and repeated subsequently for up to 32 days postinjury. A cohort of mice not exposed to the nerve crush procedure served as control. En face SD-OCT images were used to manually align subsequent scans to the baseline en face image. Total retinal thickness (TRT) (along a sampling band with radii 0.33-0.42 mm centered on the ONH) from each follow-up day was automatically quantified for global and sectoral measurements using custom software. Linear mixed-effects models with quadratic terms were fitted to compare TRT of nerve-crushed and control eyes over time.RESULTS. Eleven eyes from 11 nerve crush mice (baseline age 76 +/- 11.8 days) and eight eyes from four healthy mice (baseline age 64 +/- 0 days) were included. The control eyes showed a small, gradual, and consistent TRT increase throughout follow-up. Nerve-crushed eyes showed an initial period of thickening, followed by thinning and slight rebound after day 21. The decrease in thickness observed after the early thickening resolved was statistically significantly different from the control eyes (P < 0.05 for global and sectoral measurements).CONCLUSIONS. SD-OCT can be used to quantitatively monitor changes in retinal thickness in mice over time. (Invest Ophthalmol Vis Sci. 2011;52:2250-2254) DOI:10.1167/iovs.106311