Internalization and cytotoxicity analysis of silicon-based microparticles in macrophages and embryos
Internalization and cytotoxicity analysis of silicon-based microparticles in macrophages and embryos
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DOI：
10.1007/s10544-009-9393-6
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发表时间：
2010-06-01
影响因子：
2.8
通讯作者：
Nogues, Carme
中科院分区：
文献类型：
--
作者：
Fernandez-Rosas, Elisabet;Gomez, Rodrigo;Nogues, Carme
利用半导体技术，微芯片可以在微观水平上被制造出来，并被引入活细胞中，用于在单个细胞水平上监测细胞内参数。作为细胞内芯片开发的第一步，制备了形状和尺寸可控的硅和多晶硅微粒，并分别通过吞噬和显微注射引入人巨噬细胞和小鼠胚胎。微颗粒对巨噬细胞无细胞毒性，主要定位于早期核内体，与核内体膜紧密相关，很少出现在酸性腔室中。注射微颗粒的胚胎正常发育至囊胚期，证实了微颗粒的无细胞毒性作用。鉴于这些结果，硅和多晶硅微粒可以作为未来细胞内芯片开发的框架，这项技术开启了真正复杂的设备被用作活细胞内传感器或执行器的可能性。
Microchips can be fabricated, using semiconductor technologies, at microscopic level to be introduced into living cells for monitoring of intracellular parameters at a single cell level. As a first step towards intracellular chips development, silicon and polysilicon microparticles of controlled shape and dimensions were fabricated and introduced into human macrophages and mouse embryos by phagocytosis and microinjection, respectively. Microparticles showed to be non-cytotoxic for macrophages and were found to be localized mainly inside early endosomes, in tight association with endosomal membrane, and more rarely in acidic compartments. Embryos with microinjected microparticles developed normally to the blastocyst stage, confirming the non-cytotoxic effect of the particles. In view of these results silicon and polysilicon microparticles can serve as the frame for future intracellular chips development and this technology opens the possibility of real complex devices to be used as sensors or actuators inside living cells.