NATURAL CYTOTOXIC REACTIVITY OF MOUSE LYMPHOID-CELLS AGAINST SYNGENEIC AND ALLOGENEIC TUMORS .1. DISTRIBUTION OF REACTIVITY AND SPECIFICITY
NATURAL CYTOTOXIC REACTIVITY OF MOUSE LYMPHOID-CELLS AGAINST SYNGENEIC AND ALLOGENEIC TUMORS .1. DISTRIBUTION OF REACTIVITY AND SPECIFICITY
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DOI：
10.1002/ijc.2910160204
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发表时间：
1975-01-01
影响因子：
6.4
通讯作者：
LAVRIN, DH
中科院分区：
文献类型：
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作者：
HERBERMAN, RB;NUNN, ME;LAVRIN, DH
在 51Cr 释放细胞毒性测定中，发现来自多种近交系的许多正常小鼠的淋巴细胞对几种同基因和同种异体肿瘤具有反应性。来自无胸腺裸鼠的效应细胞具有非常高的反应性，这与反应性不依赖于 T 细胞的其他证据一致。易裂解的靶细胞包括由致癌C型病毒诱导的肿瘤，也包括由其他方式诱导的表达内源C型病毒的肿瘤。自然反应水平受年龄影响，5 至 8 周龄小鼠的细胞产生的细胞毒性最高。淋巴结细胞、脾细胞、腹膜渗出细胞和外周血淋巴细胞均具有细胞毒性反应性。通过抑制测定详细分析反应的特异性。获得了针对几种不同抗原的天然反应性的证据，每种抗原显然与鼠内源性 C 型病毒的表达相关。
Lymphoid cells from many normal mice of a variety of inbred strains were found to have reactivity, in a51Cr release cytotoxicity assay, against several syngeneic and allogeneic tumors. Very high reactivity was seen with effector cells from athymic nude mice, which was consistent with other evidence that the reactivity was not T‐cell dependent. Target cells susceptible to lysis included tumors induced by oncogenic type‐C viruses but also tumors induced by other means and expressing endogenous type‐C viruses. The levels of natural reactivity were influenced by age, with highest cytotoxicity produced by cells from 5‐ to 8‐week‐old mice. Lymph‐node cells, spleen cells, peritoneal exudate cells and peripheral blood lymphocytes all had cytotoxic reactivity. The specificity of the reactions was analyzed in detail by an inhibition assay. Evidence was obtained for natural reactivity against several different antigens, each apparently associated with expression of murine endogenous type‐C viruses.