Using C. elegans to screen for targets of ethanol and behavior-altering drugs.
Using C. elegans to screen for targets of ethanol and behavior-altering drugs.
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DOI：
10.1251/bpo79
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发表时间：
2004
影响因子：
6.4
通讯作者：
McIntire SL
中科院分区：
文献类型：
--
作者：
Davies AG;McIntire SL
Caenorhabditis elegans is an attractive model system for determining the targets of neuroactive compounds. Genetic screens in C. elegans provide a relatively unbiased approach to the identification of genes that are essential for behavioral effects of drugs and neuroactive compounds such as alcohol. Much work in vertebrate systems has identified multiple potential targets of ethanol but which, if any, of those candidates are responsible for the behavioral effects of alcohol is uncertain. Here we provide detailed methodology for a genetic screen for mutants of C. elegans that are resistant to the depressive effects of ethanol on locomotion and for the subsequent behavioral analysis of those mutants. The methods we describe should also be applicable for use in screening for mutants that are resistant or hypersensitive to many neuroactive compounds and for identifying the molecular targets or biochemical pathways mediating drug responses.
