A cost-effective fluorescence mini-microscope for biomedical applications.
A cost-effective fluorescence mini-microscope for biomedical applications.
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DOI：
10.1039/c5lc00666j
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发表时间：
2015
期刊：
影响因子：
6.1
通讯作者：
Khademhosseini A
中科院分区：
文献类型：
--
作者：
Zhang YS;Ribas J;Nadhman A;Aleman J;Selimović Š;Lesher-Perez SC;Wang T;Manoharan V;Shin SR;Damilano A;Annabi N;Dokmeci MR;Takayama S;Khademhosseini A
我们已经设计和制造了一个微型显微镜从现成的组件和网络摄像头，内置荧光功能的生物医学应用。微型显微镜能够检测生物化学参数如细胞/组织活力（例如活/死测定）和微环境的生物物理性质如基于氧敏感荧光染料的微制造组织中的氧水平。这种微型显微镜具有8- 60倍的可调放大倍率，分辨率高达<2 μm，并且具有4.5 mm的长工作距离（放大倍率为8倍）。微型显微镜能够按时间顺序监测细胞迁移，并分别以真实的时间分析微流体肝脏和心脏生物反应器的跳动。微型显微镜系统是便宜的，并且其模块化允许方便地与各种各样的预先存在的平台集成，包括但不限于细胞培养板、微流体装置和芯片上器官系统。因此，我们设想其在细胞生物学，组织工程，生物传感，微流体和器官芯片中的广泛应用，这可能会取代传统的台式显微镜，需要长期原位和大规模成像/分析。
We have designed and fabricated a miniature microscope from off-the-shelf components and webcam, with built-in fluorescence capability for biomedical applications. The mini-microscope was able to detect both biochemical parameters such as cell/tissue viability (e.g. Live/Dead assay), and biophysical properties of the microenvironment such as oxygen levels in microfabricated tissues based on an oxygen-sensitive fluorescent dye. This mini-microscope has adjustable magnifications from 8-60X, achieves a resolution as high as <2 μm, and possesses a long working distance of 4.5 mm (at a magnification of 8X). The mini-microscope was able to chronologically monitor cell migration and analyze beating of microfluidic liver and cardiac bioreactors in real time, respectively. The mini-microscope system is cheap, and its modularity allows convenient integration with a wide variety of pre-existing platforms including but not limited to, cell culture plates, microfluidic devices, and organs-on-a-chip systems. Therefore, we envision its widespread applications in cell biology, tissue engineering, biosensing, microfluidics, and organs-on-chips, which can potentially replace conventional bench-top microscopy where long-term in situ and large-scale imaging/analysis is required.