Cell-sensitive phase contrast microscopy imaging by multiple exposures
Cell-sensitive phase contrast microscopy imaging by multiple exposures
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通过多次曝光进行细胞敏感相差显微镜成像
DOI：
10.1016/j.media.2015.04.011
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发表时间：
2015
影响因子：
10.9
通讯作者：
Haohan Li
中科院分区：
文献类型：
--
作者：
Zhaozheng Yin;Hang Su;Dai Fei Elmer Ker;Mingzhong Li;Haohan Li
提出了一种利用相差显微镜对培养皿中的活细胞进行成像的新方法。通过在同一细胞培养皿上多次曝光相衬显微镜图像，我们估计了一个细胞敏感的相机响应函数，该函数响应细胞的辐射信号，但在非细胞背景信号上产生一个常数。这种新的显微成像的结果是视觉上质量优越，它揭示了细胞的外观细节，并将背景噪声抑制在接近零的水平。使用细胞敏感显微镜成像，从所有曝光中恢复细胞的原始辐射信号，并将非细胞背景区域的辐射信号恢复为一个统一的常数(即成像系统仅对细胞敏感，但对非细胞背景不敏感)。通过简单的阈值分割，恢复的辐照度信号极大地方便了细胞分割。实验结果表明，该方法可以实现高质量的细胞分割。
We propose a novel way of imaging live cells in a Petri dish by the phase contrast microscope. By taking multiple exposures of phase contrast microscopy images on the same cell dish, we estimate a cell-sensitive camera response function which responds to cells’ irradiance signals but generates a constant on non-cell background signal. The result of this new microscopy imaging is visually superior quality, which reveals the appearance details of cells and suppresses background noise near zero. Using the cell-sensitive microscopy imaging, cells’ original irradiance signals are restored from all exposures and the irradiance signals on non-cell background regions are restored as a uniform constant (i.e., the imaging system is sensitive to cells only but insensitive to non-cell background). The restored irradiance signals greatly facilitate the cell segmentation by simple thresholding. The experimental results validate that high quality cell segmentation can be achieved by our approach.