GABAERGIC PROJECTION FROM THE BASAL FOREBRAIN TO THE VISUAL SECTOR OF THE THALAMIC RETICULAR NUCLEUS IN THE CAT
GABAERGIC PROJECTION FROM THE BASAL FOREBRAIN TO THE VISUAL SECTOR OF THE THALAMIC RETICULAR NUCLEUS IN THE CAT
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DOI：
10.1002/cne.903480402
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发表时间：
1994-10-22
影响因子：
2.5
通讯作者：
SHERMAN, SM
中科院分区：
文献类型：
--
作者：
BICKFORD, ME;GUNLUK, AE;SHERMAN, SM
我们研究了投射从基底前脑丘脑和皮质区域的视觉系统在猫，特别是参考的视觉部门的丘脑网状核，外侧膝状体核，和纹状体皮质。首先，我们将麦胚凝集素结合辣根过氧化物酶（WGA-HRP）注射到丘脑网状核的视觉部分，并发现在外侧基底核大细胞逆行转运标记的细胞。注射biocytin到基底前脑导致在整个丘脑网状核内的纤维和终端的致密带顺行标记，这种标签延伸通过视觉部门，包括膝状体周围核。外侧膝状体核内未见顺行标记。接下来，我们使用各种免疫细胞化学和组织化学标记物来解决这个通路所使用的假定神经递质的问题。以这种方式，我们确定了两个群体的细胞在基底核大细胞的猫：大胆碱能细胞，含有胆碱乙酰转移酶，NADPH-黄递酶，钙结合蛋白和项目的纹状体皮质和较小的细胞，含有γ-氨基丁酸（GABA），谷氨酸脱羧酶，和小白蛋白和项目的视觉部门的丘脑网状核。我们还研究了在电子显微镜水平的终端在视觉部门的丘脑网状核标记从生物胞素注射在基底前脑。大多数这些终端形成对称的接触树突，并揭示了由postembedding免疫细胞化学染色阳性GABA。(C)1994 Wiley-Liss，Inc.
We examined the projection from the basal forebrain to thalamic and cortical regions of the visual system in cats, with particular reference to the visual sector of the thalamic reticular nucleus, the lateral geniculate nucleus, and the striate cortex. First, we made injections of wheat germ agglutinin conjugated to horseradish peroxidase (WGA-HRP) into the visual sector of the thalamic reticular nucleus and found cells labeled by retrograde transport in the lateral nucleus basalis magnocellularis. Injection of biocytin into the basal forebrain resulted in the anterograde labeling of a dense band of fibers and terminals within the entire thalamic reticular nucleus; this labeling extended through the visual sector including the perigeniculate nucleus. No orthograde labeling was found in the lateral geniculate nucleus. Next, we addressed the issue of putative neurotransmitters used by this pathway using a variety of immunocytochemical and histochemical markers. In this fashion, we identified two populations of-cells in the nucleus basalis magnocellularis of the cat: large cholinergic cells that contain choline acetyltransferase, NADPH-diaphorase, and calbindin and that project to striate cortex-and smaller cells that contain gamma-aminobutyric acid (GABA), glutamic acid decarboxylase, and parvalbumin and that project to the visual sector of the thalamic reticular nucleus. We also examined at the electron microscopic level terminals in the visual sector of the thalamic reticular nucleus that were labeled from a biocytin injection in the basal forebrain. Most of these terminals form symmetric contacts onto dendrites and were revealed by postembedding immunocytochemical staining to be positive for GABA. (C) 1994 Wiley-Liss, Inc.