Polyacrylonitrile nanofibrous mat from electrospinning: Born with potential anti-fungal functionality
Polyacrylonitrile nanofibrous mat from electrospinning: Born with potential anti-fungal functionality
复制标题
静电纺丝聚丙烯腈纳米纤维垫：天生具有潜在的抗真菌功能
DOI：
10.1016/j.eurpolymj.2019.07.035
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发表时间：
2019
影响因子：
6
通讯作者：
Zhang, Lifeng
中科院分区：
文献类型：
--
作者：
Sirelkhatim, Nafisa;Parveen, Arifa;LaJeunesse, Dennis;Yu, Demei;Zhang, Lifeng
电纺纳米纤维已在空气/水过滤、高性能服装、药物输送、组织工程支架等领域得到应用，并开始集成到商业产品中，从而使其暴露在环境中。电纺纳米纤维材料对于自然界中的微生物来说是一种相对较新的材料，对电纺纳米纤维垫与细胞真菌细胞之间相互作用的生物学意义知之甚少。本文研究了静电纺丝聚丙烯腈(ESPAN)纳米纤维垫与具有代表性的非致病/致病细胞酵母(酿酒酵母和白色念珠菌)之间的相互作用。首次证明，在没有任何化学抗真菌药物存在的情况下，当这些细胞酵母菌暴露在ESPAN纳米纤维垫子中时，它们的生长速度较慢，形态发生了根本性的变化，活力降低。这些反应不同于细胞与其他形式的PAN材料(例如，固体薄膜或微纤维垫)的相互作用。对机理的探讨表明，酵母细胞与电纺纳米纤维毡的相互作用是一个复杂的现象，其中纳米纤维的形态和纤维表面的组成/性质都起着重要作用。ESPAN纳米纤维垫固有的抗酵母和潜在的抗真菌功能可能会对环境微生物产生直接影响，也可能有利于下一代材料设计，通过仅通过物理接触来控制微生物生长。
Electrospun nanofibers have been found in many applications such as air/water filtration, performance apparel, drug delivery, and scaffold for tissue engineering and started to be integrated in commercial products, which leads to their exposure to environment. Electrospun nanofibrous material is a relatively new material to microorganism in nature and little is known about the biological implication of interactions between electrospun nanofibrous mats and cellular fungal cells. Herein the interaction between electrospun polyacrylonitrile (ESPAN) nanofibrous mat and representative non-pathogenic/pathogenic cellular yeasts (Saccharomyces cerevisiaeandCandida albicans) was investigated. It is demonstrated for the first time that when these cellular yeasts, species of the kingdom fungi, were exposed to ESPAN nanofibrous mat, they exhibited lower growth rate, radical change to morphology, and reduced viability without presence of any chemical antifungal agent. These responses were distinct from the cellular interactions with other forms of PAN materials (e.g. solid film or microfibrous mat). Exploration of mechanism indicated that the interaction between yeast cell and electrospun nanofibrous mat is a complex phenomenon in which both nanofibrous morphology and fiber surface composition/property play significant roles. The inherent anti-yeast and potential anti-fungal functionality of ESPAN nanofibrous mat may make an immediate impact on environmental microorganism and could also benefit the next-generation material design to control microbial growth through solely physical contact.