Elevated zinc induces siderophore biosynthesis genes and a zntA-like gene in Pseudomonas fluorescens.
Elevated zinc induces siderophore biosynthesis genes and a zntA-like gene in Pseudomonas fluorescens.
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DOI：
10.1111/j.1574-6968.2000.tb09320.x
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发表时间：
2000-10
影响因子：
2.1
通讯作者：
Silvia Rossbach;Thomas L. Wilson;M. L. Kukuk;Heather A Carty
中科院分区：
文献类型：
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作者：
Silvia Rossbach;Thomas L. Wilson;M. L. Kukuk;Heather A Carty
采用报告基因转座子突变的方法对荧光假单胞菌锌调控基因进行分析。六个突变体对锌浓度升高的反应是基因表达增加。遗传和生化分析表明，在六个突变体中的四个中，转座子插入了铁载体吡喃二烯生物合成所必需的基因。其中一个突变体的生长在镉和锌离子浓度升高的情况下受到严重损害。在这个突变体中，转座子插入了一个与参与锌和镉离子转运的P型ATPase高度相似的基因。四个突变体对锌浓度升高的反应降低了基因表达。其中一个突变株对锌、镉和铜离子敏感。这个突变体的目标基因区域与任何已知基因都没有显示出相似之处。
Zinc-regulated genes were analyzed in Pseudomonas fluorescens employing mutagenesis with a reporter gene transposon. Six mutants responded with increased gene expression to elevated concentrations of zinc. Genetic and biochemical analysis revealed that in four of the six mutants the transposon had inserted into genes essential for the biosynthesis of the siderophore pyoverdine. The growth of one of the mutants was severely impaired in the presence of elevated concentrations of cadmium and zinc ions. In this mutant, the transposon had inserted in a gene with high similarity to P-type ATPases involved in zinc and cadmium ion transport. Four mutants reacted with reduced gene expression to elevated concentrations of zinc. One of these mutants was sensitive to zinc, cadmium and copper ions. The genetic region targeted in this mutant did not show similarity to any known gene.