A simple method to mass produce monospores in the thallus of Porphyra yezoensis Ueda
A simple method to mass produce monospores in the thallus of Porphyra yezoensis Ueda
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DOI：
10.1023/a:1025170010916
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发表时间：
2003-07-01
影响因子：
3.3
通讯作者：
Saga, N
中科院分区：
文献类型：
--
作者：
Mizuta, H;Yasui, H;Saga, N
本文介绍了一种从条斑紫菜叶状体中获得大量单孢子的简便方法。该方法包含两个原则：尿囊素诱导单孢子囊形成，和通过温和的均质化从细胞壁释放单孢子。单孢子囊形成的诱导是通过在富含营养的海水中用10 mM尿囊素培养野生菌体约3周来完成的。与较低浓度（0-1 mM）相比，该高浓度（10 mM）的尿囊素抑制了菌体的生长。将培养3周的菌体用玻璃匀浆器温和匀浆，并通过用尼龙网过滤获得单孢子溶液。单孢子正常生长至菌体。该单孢子获得技术是条斑紫菜叶状体繁殖和育种的一种简单有效的方法。
A simple method is presented for obtaining a large number of monospores in the thallus of Porphyra yezoensis. The method implies two principles: induction of monosporangium formation by allantoin, and liberation of monospores from the cell wall by mild homogenization. The induction of monosporangium formation was accomplished by culturing wild thalli in nutrient-enriched seawater with 10 mM of allantoin for approximately 3 weeks. This high concentration (10 mM) of allantoin suppressed the growth of the thalli compared with lower concentrations (0-1 mM). Thalli cultured for 3 weeks were mildly homogenized with a glass homogenizer and the monospore solution was obtained by filtering with a nylon mesh. The monospores grew normally to thalli. This technique of monospore acquisition is a simple and useful method for the propagation and breeding of P. yezoensis thalli.