Regulation of phosphoenolpyruvate carboxykinase (GTP) in adipose tissue in vivo by glucocorticoids and insulin.
Regulation of phosphoenolpyruvate carboxykinase (GTP) in adipose tissue in vivo by glucocorticoids and insulin.
复制标题
糖皮质激素和胰岛素对体内脂肪组织中磷酸烯醇丙酮酸羧激酶（GTP）的调节。
DOI：
10.1042/bj1580001
复制
发表时间：
1976
期刊：
影响因子：
--
通讯作者：
F. Ballard
中科院分区：
文献类型：
--
作者：
O. Meyuhas;L. Reshef;J. Gunn;R. Hanson;F. Ballard
1.免疫化学研究了体内附睾脂肪组织、肝脏和肾脏中磷酸烯醇丙酮酸羧激酶(GTP)(EC 4.1.1.32)合成的调节。 2.脂肪组织中磷酸烯醇丙酮酸羧激酶（GTP）的合成因饥饿、糖尿病和去甲肾上腺素而增加，而因重新进食和胰岛素而减少。这些变化也在肾上腺切除的大鼠中观察到，并且在性质上与肝酶观察到的变化相似。这表明环 AMP 作为诱导剂和胰岛素作为去诱导剂参与两种组织中磷酸烯醇丙酮酸羧激酶 (GTP) 的调节。 （诱导和去诱导分别定义为酶合成速率的选择性增加和减少，无论所涉及的机制如何。）3。肾上腺切除术对肝脏和肾脏中磷酸烯醇丙酮酸羧激酶（GTP）合成影响不大，但增加了脂肪组织酶的合成率。饥饿和肾上腺切除术在增加脂肪组织磷酸烯醇丙酮酸羧激酶（GTP）的合成率方面具有累加效应。在肾上腺切除的糖尿病大鼠中，糖皮质激素增加了肝脏和肾脏中磷酸烯醇丙酮酸羧激酶（GTP）的合成，同时减少了脂肪组织中酶的合成。因此，脂肪组织磷酸烯醇式丙酮酸羧激酶（GTP）的去诱导受到糖皮质激素和胰岛素的独立调节。 4. 尽管肝脏、肾脏和脂肪组织的磷酸烯醇丙酮酸羧激酶 (GTP) 看似相同，但该酶的调节系统存在明显的组织特异性差异。
1. The regulation of the synthesis of phosphoenolpyruvate carboxykinase (GTP) (EC 4.1.1.32) in epididymal adipose tissue, liver and kidney in vivo was studied immunochemically. 2. Phosphoenolpyruvate carboxykinase (GTP) synthesis in adipose tissue is increased by starvation, diabetes and noradrenaline, and decreased by re-feeding and insulin. These changes were also seen in adrenalectomized rats and are qualitatively similar to those observed for the liver enzyme. This indicates the involvement of cyclic AMP as an inducer and insulin as a de-inducer in the regulation of phosphoenolpyruvate carboxykinase (GTP) in both tissues. (Induction and de-induction are defined as selective increase and decrease respectively in the rate of enzyme synthesis, regardless of the mechanism involved.)3. Adrenalectomy had little effect on phosphoenolpyruvate carboxykinase (GTP) synthesis in liver and kidney, but increased the synthesis rate of the adipose-tissue enzyme. Starvation and adrenalectomy had additive effects in increasing the synthesis rate of adipose-tissue phosphoenolpyruvate carboxykinase (GTP). In adrenalectomized diabetic rats glucocorticoids increased phosphoenolpyruvate carboxykinase (GTP) synthesis in liver and kidney while decreasing enzyme synthesis in adipose tissue. De-induction of adipose tissue phosphoenolpyruvate carboxykinase (GTP) is therefore regulated independently by glucocorticoids and insulin. 4. Although liver, kidney and adipose-tissue phosphoenolpyruvate carboxykinases (GTP) are seemingly identical, there is an apparent tissue-specific differentiation in regulatory systems for the enzyme.