A genome-wide study of gene activity reveals developmental signaling pathways in the preimplantation mouse embryo
A genome-wide study of gene activity reveals developmental signaling pathways in the preimplantation mouse embryo
复制标题
DOI：
10.1016/s1534-5807(03)00404-0
复制
发表时间：
2004-01-01
影响因子：
11.8
通讯作者：
Zernicka-Goetz, M
中科院分区：
文献类型：
--
作者：
Wang, QT;Piotrowska, K;Zernicka-Goetz, M
哺乳动物胚胎的植入前发育包含一系列关键事件：从卵母细胞到胚胎的转变、第一次细胞分裂、细胞接触的建立、第一次谱系分化——所有朝着未来身体计划迈出的第一个微妙步骤。在这里，我们使用微阵列；探索植入前发育过程中的基因活性。我们揭示了阶段特异性基因活性的稳健和动态模式，该模式分为两个主要阶段，一个是 2 细胞阶段（卵母细胞到胚胎转变），另一个是 4 细胞阶段（细胞分化）之后。小鼠卵母细胞和早期胚胎表达多种信号通路的成分，包括 Wnt、BMP 和 Notch 下游的信号通路，表明细胞命运和模式形成的保守调节因子可能在最早的胚胎阶段发挥作用。总体而言，这些数据提供了基因表达的详细时间概况，揭示了早期哺乳动物发育中信号传导过程的丰富性。
The preimplantation development of the mammalian embryo encompasses a series of critical events: the transition from oocyte to embryo, the first cell divisions, the establishment of cellular contacts, the first lineage differentiation - all the first subtle steps toward a future body plan. Here, we use microarrays; to explore gene activity during preimplantation development. We reveal robust and dynamic patterns of stage-specific gene activity that fall into two major phases, one up to the 2-cell stage (oocyte-to-embryo transition) and one after the 4-cell stage (cellular differentiation). The mouse oocyte and early embryo express components of multiple signaling pathways including those downstream of Wnt, BMP, and Notch, indicating that conserved regulators of cell fate and pattern formation are likely to function at the earliest embryonic stages. Overall, these data provide a detailed temporal profile of gene expression that reveals the richness of signaling processes in early mammalian development.