Efficacy and putative mode of action of native and commercial antagonistic yeasts against postharvest pathogens of pear
Efficacy and putative mode of action of native and commercial antagonistic yeasts against postharvest pathogens of pear
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DOI：
10.1016/j.ijfoodmicro.2013.04.005
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发表时间：
2013-06-17
影响因子：
5.4
通讯作者：
Sangorrin, Marcela P.
中科院分区：
文献类型：
--
作者：
Cecilia Lutz, M.;Lopes, Christian A.;Sangorrin, Marcela P.
采用体外和原位测定方法，研究了4株酵母菌(白色隐球菌NPCC1248、膜面毕赤酵母NPCC1250、维氏隐球菌NPCC1263和NPCC1259)对扩展青霉和灰霉病菌的生防作用机理。白化葡萄球菌(Yp)，一种商业酵母，也被评估用于比较目的。酵母菌株表现出多种不同的机制，包括：伤口定植、萌发抑制、生物膜形成、杀伤性毒素的分泌、营养竞争和水解酶(蛋白酶、几丁质酶和葡聚糖酶)的分泌。还对菌株(及其相关的拮抗机制)与现场拮抗活性的关系进行了评估。结果表明，水解酶的产生、伤口定植能力、杀伤毒素的产生和萌发抑制等机制是影响生防活性的重要因素。我们的研究表明，多种作用模式可能解释了为什么膜面青霉NPCC1250和黄曲霉NPCC1263对梨采后病害有很好的控制作用。(C)2013爱思唯尔B.V.保留所有权利。
Putative mechanisms of action associated with the biocontrol capacity of four yeast strains (Cryptoccocus albidus NPCC 1248, Pichia membranifaciens NPCC 1250, Cryptoccocus victoriae NPCC 1263 and NPCC 1259) against Penicillium expansum and Botrytis cinerea were studied by means of in vitro and in situ assays. C. albidus((YP)), a commercial yeast was also evaluated for comparative purposes. The yeast strains exhibited a variety of different mechanisms including: wound colonization, germination inhibition, biofilm formation, secretion of killer toxins, competition for nutrient and secretion of hydrolytic enzymes (protease, chitinase and glucanase). The relationship between strains (and their associated antagonist mechanisms) and in situ antagonist activity was also evaluated. Results indicate that mechanisms such as production of hydrolytic enzymes, the ability for colonization of wounds, production of killer toxin and inhibition of germination are the most important for biocontrol activity. Our study indicate that multiple modes of action may explain why P. membranifaciens NPCC 1250 and C. victoriae NPCC 1263 provided excellent control of postharvest pears disease. (c) 2013 Elsevier B.V. All rights reserved.