Cloning and expression analysis of chalcone synthase gene from Coleus forskohlii
Cloning and expression analysis of chalcone synthase gene from Coleus forskohlii
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DOI：
10.1007/s12041-016-0680-8
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发表时间：
2016-09-01
影响因子：
1.5
通讯作者：
Gandhi, Sumit G.
中科院分区：
文献类型：
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作者：
Awasthi, Praveen;Mahajan, Vidushi;Gandhi, Sumit G.
黄酮类化合物是一类重要的次生代谢产物，在植物体内起着多种作用，如介导植物的防御、花的着色以及植物与微生物的相互作用。还已知黄酮类化合物具有抗氧化剂和抗微生物活性。毛喉鞘蕊花Coleus forskohlii（Willd.）Briq.（唇形科）是一种重要的药用植物，具有多种代谢特征，包括产生黄酮类化合物芫花素。然而，尚未在这种植物中研究类黄酮途径的组分。查耳酮合酶（CHS）是黄酮类化合物生物合成途径中的第一个关键步骤。通过克隆和测序，获得了与植物CHS基因同源的全长cDNA。forskohlii，命名为CfCHS（GenBank登录号KF643243）。CfCHS基因编码391个氨基酸，分子量为42.75 kDa，等电点为6.57。CfCHS在不同组织和诱导子处理中的表达分析表明，茉莉酸甲酯（MeJA）强烈诱导其表达。结果表明，不同提取工艺下，毛果芸香总黄酮含量及抗氧化活性均高于对照组Forskohlii对MeJA的反应也得到增强，这与CfCHS表达的增加相关。MeJA对CfCHS的诱导表明其参与类黄酮的产生，在食草动物或机械创伤期间提供免受微生物侵害的保护。此外，我们的计算机模拟预测和实验数据表明，CfCHS可能是转录后调控的miR 34。
Flavonoids are an important class of secondary metabolites that play various roles in plants such as mediating defense, floral pigmentation and plant-microbe interaction. Flavonoids are also known to possess antioxidant and antimicrobial activities. Coleus forskohlii (Willd.) Briq. (Lamiaceae) is an important medicinal herb with a diverse metabolic profile, including production of a flavonoid, genkwanin. However, components of the flavonoid pathway have not yet been studied in this plant. Chalcone synthase (CHS) catalyses the first committed step of flavonoid biosynthetic pathway. Full-length cDNA, showing homology with plant CHS gene was isolated from leaves of C. forskohlii and named CfCHS (GenBank accession no. KF643243). Theoretical translation of CfCHS nucleotide sequence shows that it encodes a protein of 391 amino acids with a molecular weight of 42.75 kDa and pI 6.57. Expression analysis of CfCHS in different tissues and elicitor treatments showed that methyl jasmonate (MeJA) strongly induced its expression. Total flavonoids content and antioxidant activity of C. forskohlii also got enhanced in response to MeJA, which correlated with increased CfCHS expression. Induction of CfCHS by MeJA suggest its involvement in production of flavonoids, providing protection from microbes during herbivory or mechanical wounding. Further, our in silico predictions and experimental data suggested that CfCHS may be posttranscriptionally regulated by miR34.