Surface motion upregulates superficial zone protein and hyaluronan production in chondrocyte-seeded three-dimensional scaffolds
Surface motion upregulates superficial zone protein and hyaluronan production in chondrocyte-seeded three-dimensional scaffolds
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DOI：
10.1089/ten.2005.11.249
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发表时间：
2005-01-01
影响因子：
--
通讯作者：
Alini, M
中科院分区：
文献类型：
--
作者：
Grad, S;Lee, CR;Alini, M
已经开发了一种可提供联合特异性运动学的软骨工程生物反应器。这项研究研究了关节运动对浅层区蛋白（SZP）和透明质酸合酶（HASS）的基因表达的影响，以及软骨细胞的SZP和透明质酸释放到可生物降解的支架上。将圆柱形（8 x 4 mm）的多孔聚氨酯支架用牛关节软骨细胞接种，并受到静态或动态压缩的作用，并没有针对陶瓷髋关节的表现。加载后，分析了SZP和HASS的mRNA表达，并确定了条件培养基的SZP免疫反应性和透明质酸浓度。表面运动显着上调了SZP和HASS的mRNA表达。仅轴向压缩对SZP没有影响，并且增加仅在高应变幅度下mRNA。 SZP仅在暴露于表面运动的构造的介质中进行免疫进行。透明质酸将透明质酸释放到培养基中，通过表面运动显着增强。这些结果表明，模仿自然关节运动学的特定刺激（例如关节运动）可能会促进功能性关节表面 - 滑膜界面的发展。
A cartilage engineering bioreactor has been developed that provides joint-specific kinematics. This study investigated the effect of articular motion on the gene expression of superficial zone protein (SZP) and hyaluronan synthases (HASs) and on the release of SZP and hyaluronan of chondrocytes seeded onto biodegradable scaffolds. Cylindrical ( 8 x 4 mm) porous polyurethane scaffolds were seeded with bovine articular chondrocytes and subjected to static or dynamic compression, with and without articulation against a ceramic hip ball. After loading, the mRNA expression of SZP and HASs was analyzed, and SZP immunoreactivity and hyaluronan concentration of conditioned media were determined. Surface motion significantly upregulated the mRNA expression of SZP and HASs. Axial compression alone had no effect on SZP and increased HAS mRNA only at high strain amplitude. SZP was immunodetected only in the media of constructs exposed to surface motion. The release of hyaluronan into the culture medium was significantly enhanced by surface motion. These results indicate that specific stimuli that mimic the kinematics of natural joints, such as articular motion, may promote the development of a functional articular surface - synovial interface.