REVERSAL BY COMPLEX NATURAL MATERIALS OF GROWTH INHIBITION CAUSED BY ANTIBIOTICS
REVERSAL BY COMPLEX NATURAL MATERIALS OF GROWTH INHIBITION CAUSED BY ANTIBIOTICS
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DOI：
10.1128/jb.65.4.361-367.1953
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发表时间：
1953-01-01
影响因子：
3.2
通讯作者：
PITTILLO, RF
中科院分区：
文献类型：
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作者：
FOSTER, JW;PITTILLO, RF
已设计了一种扩展板法，显示了4-8倍的康宁。在小AMT存在的情况下，需要青霉素、链霉素、金霉素、氯霉素、土霉素和杆菌素的混合制剂才能完全抑制大肠杆菌。(100至1000[亩]克/毫升。)在没有有机补充剂的情况下，添加复合有机补充剂。这被解释为复杂混合物中的一种或多种物质逆转了抗生素抑制。半胱氨酸和半胱氨酸在所使用的条件下没有作用；复合材料的逆转活性不是抗生素破坏的结果。给出了适用于这一问题的扩散板法的特点，并描述了抗生素抑制的一些特点。讨论了液体培养对这类工作的不足之处。
A spread plate procedure has been devised to show that 4-8 times the concn. of penicillin, streptomycin, aureomycin, chloramphenicol, terramycin, and bacillin are required for complete inhibition of Escherichia coli in the presence of small amts. (100 to 1000 [mu]g./ml.) of complex organic supplements than in their absence. This is interpreted as a reversal of antibiotic inhibition by one or more substances in the complex mixtures. Cysteine and cystine had no effect under the conditions employed; reversal activity of the complex materials is not the result of destruction of the antibiotic. Characteristics of the spread plate procedure as applied to this problem are given, and some features of the counteraction of inhibition by antibiotics are descr. Inadequacies of liquid cultures for work of this kind are discussed.