HISTONE MESSENGER-RNAS CONTAIN BLOCKED AND METHYLATED 5' TERMINAL SEQUENCES BUT LACK METHYLATED NUCLEOSIDES AT INTERNAL POSITIONS
HISTONE MESSENGER-RNAS CONTAIN BLOCKED AND METHYLATED 5' TERMINAL SEQUENCES BUT LACK METHYLATED NUCLEOSIDES AT INTERNAL POSITIONS
复制标题
DOI：
10.1016/0092-8674(77)90145-3
复制
发表时间：
1977-01-01
期刊：
影响因子：
64.5
通讯作者：
BAGLIONI, C
中科院分区：
文献类型：
--
作者：
MOSS, B;GERSHOWITZ, A;BAGLIONI, C
Histone mRNA, labeled with 32P or 3H-methionine during the S phase of partially synchronized HeLa [human cervical carcinoma] cells, was isolated from the polyribosomes and purified as a 9S component by sucrose gradient sedimentation. Two types of 5'' terminals, m7G(5'')pppNmpN and m7G(5'')pppNm-pNmpN were identified in which the first methylated nucleoside is 7-methylguanosine; the 2nd is either N6,2''-O-dimethyladenosine, 2''-O-methyladenosine or 2''-O-methylquanosine, and the 3rd is 2''-O-methyluridine, 2''-O-methylcytidine or 2''-O-methyladenosine. Approximately 1.7% of the 32P label was present in the 5'' terminal structures. Assuming a similar specific radioactivity for all phosphates, this percentage corresponds to an average of 1 terminal/335 nucleotides. Histone mRNA differed from bulk polyadenylylated mRNA of HeLa cells in lacking significant amounts of 2''-O-methyluridine or 2''-O-methylcytidine in the 2nd position of the 5'' terminal oligonucleotide and in lacking N6-methyladenosine residues at internal positions.
