CRISPR-Cas14 is now part of the artillery for gene editing and molecular diagnostic
CRISPR-Cas14 is now part of the artillery for gene editing and molecular diagnostic
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DOI：
10.1016/j.nano.2019.03.006
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发表时间：
2019-06-01
影响因子：
5.4
通讯作者：
Aquino-Jarquin, Guillermo
中科院分区：
文献类型：
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作者：
Aquino-Jarquin, Guillermo
最近，Jennifer Doudna的研究小组发现了一种规则间隔短回文重复序列（CRISPR）-CRISPR相关蛋白14（Cas 14），几乎只在嗜极古菌的一种超级真菌中发现。新发现的Cas 14具有单链（ss）DNA靶向活性-尽管比Cas9小两倍-这种能力可能赋予对ssDNA基因组病毒的防御能力。此外，通过将Cas 14的非特异性ssDNase切割活性与等温扩增方法（DETECTR-Cas 14）相结合，它也可以有希望地用于高保真DNA单核苷酸多态性基因分型，并可能用于检测感染所有三个生命领域的宿主的不可否认的临床，生态和经济重要性的ssDNA病毒。因此，CRISPR-Cas 14可能在CRISPR诊断感染性和非感染性疾病领域获得指数级扩展。(C)2019爱思唯尔公司All rights reserved.
Recently Jennifer Doudna's group discovered the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR associated protein 14 (Cas14), identified almost exclusively in a supeiphyltun of extremophile archaea. The newly discovered Cas14 possesses a single-stranded (ss)DNA targeting activity - despite being two times smaller than Cas9 - a capability that might confer a defense against viruses with ssDNA genomes. Furthermore, by combining the non-specific ssDNase cleavage activity of Cas14 with isothermal amplification method (DETECTR-Cas14), it can also be promisingly exploited for high-fidelity DNA single-nucleotide polymorphism genotyping, and potentially for detecting ssDNA viruses of undeniable clinical, ecological, and economic importance infecting hosts in all three domains of life. Thus, CRISPR-Cas14 might acquire an exponential expansion in the field of CRISPR diagnostic for infectious and noninfectious diseases. (C) 2019 Elsevier Inc. All rights reserved.