Attachment of blastocysts to lens capsule: a model system for trophoblast-epithelial cell interaction on a natural basement membrane.
Attachment of blastocysts to lens capsule: a model system for trophoblast-epithelial cell interaction on a natural basement membrane.
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囊胚与晶状体囊的附着：天然基底膜上滋养层-上皮细胞相互作用的模型系统。
DOI：
10.1007/bf00218957
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发表时间：
1987
影响因子：
3.6
通讯作者：
Wordinger,R
中科院分区：
文献类型：
--
作者：
Cammarata,PR;Oakford,L;Cantu-Crouch,D;Wordinger,R
牛透镜囊先前已显示为检查上皮细胞与基底膜的相互作用提供最佳表面。这种天然基质已被用于研究小鼠胚泡附着和滋养层细胞生长的一些初步方面。将小鼠囊胚置于前透镜囊的无细胞体液侧，孵育72 h，并通过扫描和透射电子显微镜检查。囊胚在30 h时从透明带孵化并附着。滋养层细胞在冠状方向上迅速增殖，显示出广泛的表面微绒毛，并通过许多丝状伪足的延伸而前进，其中许多丝状伪足以球根状突起终止。透射电子显微镜显示这些突起含有大量的空泡和多聚核糖体。为了进一步模拟最初的囊胚-子宫相互作用，将透镜上皮细胞悬浮液引入囊膜中，并在加入囊胚之前形成单层。在72 h时，单层透镜细胞保持完整。我们注意到：a）透镜细胞似乎从前进的滋养层细胞后退，和b）滋养层细胞在单层透镜细胞下方延伸，从而将细胞从透镜囊基质中移出。未观察到推进的滋养层细胞浸润包膜。透镜囊似乎提供了一个有前途的系统，研究滋养层上皮细胞的相互作用的天然基底膜。
The bovine lens capsule has previously been shown to provide an optimal surface for the examination of epithelial cell interaction with a basement membrane. This native substrate has been used to investigate some initial aspects of attachment of mouse blastocysts and trophoblastic cellular outgrowth. Mouse blastocysts were presented to the cell-free humoral side of the anterior lens capsule, incubated for 72 h, and examined by scanning and transmission electron microscopy. Blastocysts hatch and attach from their zonae pellucidae by 30 h. Trophoblastic cells proliferate rapidly in a coronal direction, display extensive surface microvilli, and advance by the extension of numerous filipodia, many of which terminate with bulbous projections. These projections were shown by transmission electron microscopy to contain numerous vacuoles and polysomes. To simulate further the initial blastocyst-uterine interaction, a suspension of lens epithelial cells was introduced to the capsule and permitted to form a monolayer prior to the addition of the blastocysts. At 72 h the monolayer of lens cells remained intact. We observed that: a) lens cells appear to recede from the advancing trophoblastic cells, and b) trophoblastic cells extend beneath the monolayer of lens cells and thereby dislodge the cells from the lens capsule substrate. No infiltration of the capsule by the advancing trophoblastic cells was observed. The lens capsule appears to offer a promising system for the study of trophoblast-epithelial cell interaction on a natural basement membrane.