Direct trophic effects of fibroblast growth factors on rat pancreatic acinar cells in vitro.
Direct trophic effects of fibroblast growth factors on rat pancreatic acinar cells in vitro.
复制标题
成纤维细胞生长因子对体外大鼠胰腺腺泡细胞的直接营养作用。
DOI：
10.1006/bbrc.1993.2379
复制
发表时间：
1993
影响因子：
3.1
通讯作者：
Logsdon,CD
中科院分区：
文献类型：
--
作者：
Hoshi,H;Logsdon,CD
我们研究了成纤维细胞生长因子（FGFs）对原代培养大鼠胰腺腺泡细胞的影响。碱性和酸性FGF均以剂量依赖性方式刺激[3H]胸腺嘧啶掺入。1 nM bFGF或100 nM aFGF的最大效果是对照组的6-7倍。这些数据表明，FGFs是大鼠胰腺腺泡细胞DNA合成的有效刺激物。因此，FGFs可能在体内外分泌胰腺的长期调节中发挥重要作用。我们还检测了bFGF与胰腺分泌物CCK和carbachol的相互作用。CCK本身是一种腺泡细胞营养因子，其作用与bFGF的作用是叠加的。相比之下，无生长刺激作用的碳醇不影响bFGF介导的DNA合成刺激。这些数据表明，参与腺泡细胞生长调节的机制独立于参与分泌的机制。
We examined the effects of fibroblast growth factors (FGFs) on rat pancreatic acinar cells in primary culture. Both basic and acidic FGF stimulated [3H]thymidine incorporation in a dose-dependent fashion. Maximum effects of 6-7 fold over control were seen with 1 nM bFGF or 100 nM aFGF. These data indicate that FGFs are potent stimulants of rat pancreatic acinar cell DNA synthesis. Therefore, FGFs may play an important role in long term regulation of the exocrine pancreas in vivo. We also examined the interaction of bFGF with the pancreatic secretogogues CCK and carbachol. Effects of CCK, which is itself an acinar cell trophic factor, were additive with those of bFGF. In contrast, carbachol, which has no growth stimulatory effect, did not affect bFGF mediated stimulation of DNA synthesis. These data suggest that the mechanisms involved in acinar cell growth regulation are independent from those involved in secretion.