Protease activation and the signal transduction pathway regulating motility in sperm from the water strider Aquarius remigis
Protease activation and the signal transduction pathway regulating motility in sperm from the water strider Aquarius remigis
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DOI：
10.1002/cm.21012
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发表时间：
2012-04-01
期刊：
影响因子：
2.9
通讯作者：
Cardullo, Richard A.
中科院分区：
文献类型：
--
作者：
Miyata, Haruhiko;Thaler, Catherine D.;Cardullo, Richard A.
许多运动过程是受调控的，因此运动只发生在信号级联的激活上。许多物种的精子最初是静止的，在跳动之前必须被激活。导致精子运动激活和调节的信号事件尚未得到很好的表征。来自水黾水瓶座的成熟精囊精子在体外是不动的，但活跃的运动是由胰蛋白酶激活的。胰蛋白酶激活的运动通过BAPTA-AM预处理精子以螯合细胞内Ca2+而被阻断，并通过随后添加A23187和Ca2+而部分恢复。Thapsigargin在缺乏胰蛋白酶的情况下刺激运动，表明细胞内Ca2+储存是可用的。此外，运动可以被磷酸酶抑制剂calyculin A完全激活，这表明不运动状态是由内源性磷酸酶维持的，运动需要激酶活性。MEK1/2抑制剂U0126显著降低了胰蛋白酶激活的运动性，而MPM-2（一种识别脯氨酸定向磷酸化激酶如MAPK的抗体）识别了水黾精子鞭毛的成分。小鼠蛋白酶激活受体PAR2特异性抗体识别精子鞭毛上的抗原。这些结果表明胰蛋白酶刺激Ca2+和MAPK介导的信号通路，并可能涉及par2样蛋白调节运动。(C) 2012 Wiley期刊公司
Many motile processes are regulated such that movement occurs only upon activation of a signaling cascade. Sperm from a variety of species are initially quiescent and must be activated prior to beating. The signaling events leading to the activation and regulation of sperm motility are not well characterized. Mature seminal vesicle sperm from the water strider Aquarius remigis are immotile in vitro, but vigorous motility is activated by trypsin. Trypsin-activated motility was blocked by pretreatment of the sperm with BAPTA-AM to chelate intracellular Ca2+ and was partially rescued by subsequent addition of A23187 and Ca2+. Thapsigargin stimulated motility in the absence of trypsin, suggesting that intracellular Ca2+ stores are available. In addition, motility could be fully activated by the phosphatase inhibitor calyculin A, suggesting that the immotile state is maintained by an endogenous phosphatase and that kinase activity is required for motility. The MEK1/2 inhibitor U0126 significantly reduced trypsin activated motility, and MPM-2, an antibody which recognizes proline-directed phosphorylation by kinases such as MAPK, recognized components of the water strider sperm flagellum. Antibodies specific for the mouse protease activated receptor PAR2 recognized an antigen on the sperm flagellum. These results suggest that trypsin stimulates a Ca2+ and MAPK mediated signaling pathway and potentially implicate a PAR2-like protein in regulating motility. (C) 2012 Wiley Periodicals, Inc.