A COMPARISON OF CROSSLINKING ABILITIES OF GLUTARALDEHYDE FORMALDEHYDE AND ALPHA-HYDROXYADIPALDEHYDE WITH BOVINE SERUM ALBUMIN AND CASEIN
A COMPARISON OF CROSSLINKING ABILITIES OF GLUTARALDEHYDE FORMALDEHYDE AND ALPHA-HYDROXYADIPALDEHYDE WITH BOVINE SERUM ALBUMIN AND CASEIN
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DOI：
10.1007/bf00277781
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发表时间：
1969-01-01
期刊：
影响因子：
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通讯作者：
HOPWOOD, D
中科院分区：
文献类型：
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作者：
HOPWOOD, D
用不同的方法比较了甲醛、α-羟基二醛和戊二醛的交联效果。用乙醛处理的牛血清白蛋白-兔抗牛血清白蛋白体系的微量Ouchterlone技术发现，除抗体效价很高外，戊二醛可阻止沉淀线的形成。甲醛和α-羟基二醛的影响不明显。乙醛处理的牛血清白蛋白的醋酸酯纤维素电泳示出比未经处理的蛋白的迁移率增加。乙醛固定的肝脏切片淀粉凝胶电泳法显示，戊二醛固定后蛋白质没有丢失，而其他醛允许蛋白质被提取。乙醛处理的牛血清白蛋白经甲醛和α-羟基二醛处理后，凝胶迁移率变化不大，有少量的聚合物形成。另一方面，戊二醛产生了大量的聚合物。用Sephadex G-200凝胶过滤证实了这些发现。戊二醛分子间的交联度与醛的浓度有关。
The crosslinking effects of formaldehyde, α-hydroxyadipaldehyde and glutaraldehyde have been compared by various techniques. Using a micro-Ouchterlony technique with an aldehyde treated bovine serum albumin-rabbit anti-bovine serum albumin system it was found that glutaraldehyde prevented precipitin line formation except at very high titres of antibody. The effects of formaldehyde and α-hydroxyadipaldehyde were less marked. Cellulose acetate electrophoresis of aldehyde treated bovine serum albumin showed an increase in mobility compared with the untreated protein. Starch gel electrophoresis of aldehyde fixed liver slices showed no protein loss after glutaraldehyde fixation whereas the other aldehydes permitted proteins to be extracted. Polyacrylamide gel electrophoresis of the aldehyde treated bovine serum albumin showed a little change in mobility after formaldehyde and α-hydroxyadipaldehyde treatment and a little polymer formation. Glutaraldehyde on the other hand produced much polymer. These findings were confirmed by gel filtration with Sephadex G-200. Intermolecular crosslinking with glutaraldehyde was dependant on the aldehyde concentration.