Effects of a phytosterol mixture on male fish plasma lipoprotein fractions and testis P450scc activity
Effects of a phytosterol mixture on male fish plasma lipoprotein fractions and testis P450scc activity
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DOI：
10.1016/s0016-6480(02)00590-7
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发表时间：
2003-02-01
影响因子：
2.7
通讯作者：
MacLatchy, DL
中科院分区：
文献类型：
--
作者：
Gilman, CI;Leusch, FDL;MacLatchy, DL
植物甾醇(植物甾醇)已被确定为生活在纸浆厂下游的鱼类繁殖影响的潜在来源之一。β-谷甾醇是纸浆厂废水中的主要植物类固醇，此前已被证明可以降低鱼的血浆性类固醇和胆固醇水平以及体外性腺类固醇的产生。在这项研究中，雄性鲑鱼(Salvelinus Fontinaris)和金鱼(Carassius Auratus)通过硅橡胶腹腔植入植物甾醇混合物(72%β-谷甾醇)，以帮助阐明植物甾醇对类固醇抑制的作用机制。当胆固醇被外源供应给性腺类固醇合成时，检测了血浆胆固醇组分的变化。在雄性鲑鱼中，低密度脂蛋白胆固醇和甘油三酯水平显著降低，分别为43%和50%，在植物甾醇处理的鱼中。然而，这些下降不太可能与鱼类性腺类固醇合成受抑有关。从鲑鱼或金鱼分离的睾丸线粒体中，将胆固醇转化为孕烯醇酮(类固醇合成途径的第一步)的P450scc的活性不受影响。还需要进一步研究植物甾醇的作用机制。(C)2003年埃尔塞维尔科学公司(美国)。版权所有。
Plant sterols (phytosterols) have been identified as one potential source of reproductive effects in fish living downstream of pulp mills. beta-Sitosterol, the predominant plant sterol in pulp mill effluent, has previously been shown to decrease plasma sex steroid and cholesterol levels and in vitro gonadal steroid production in fish. In this study, male brook trout (Salvelinus fontinalis) and goldfish (Carassius auratus) were exposed to a phytosterol mixture (72% beta-sitosterol) via Silastic intraperitoneal implants to help elucidate the mechanisms of action of phytosterols on steroid depression. As cholesterol is exogenously supplied for gonadal steroidogenesis, changes in plasma cholesterol fractions were examined. In male brook trout, low-density lipoprotein cholesterol and triglyceride levels decreased significantly, 43 and 50%, respectively, in phytosterol-treated fish. It is improbable, however, that these decreases are linked to depressed gonadal steroidogenesis in fish. The activity of P450scc, which converts cholesterol to pregnenolone (the first step in the steroidogenic pathway), was not affected in testis mitochondria isolated from brook trout or goldfish. Further investigation of the mechanisms of action of phytosterols is required. (C) 2003 Elsevier Science (USA). All rights reserved.