IN VITRO COMPARISON OF THE EFFECTS OF DENTAL FILLING MATERIALS ON MOUSE FIBROBLASTS
IN VITRO COMPARISON OF THE EFFECTS OF DENTAL FILLING MATERIALS ON MOUSE FIBROBLASTS
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DOI：
10.5504/bbeq.2012.0030
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发表时间：
2012-08-01
影响因子：
1.4
通讯作者：
Ozturk, Ahmet
中科院分区：
文献类型：
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作者：
Kilic, Duygu;Kesim, Servet;Ozturk, Ahmet
根管充填材料的选择是影响根管充填临床成功的重要因素。因此，必须研究填充材料的细胞毒性，以确保安全的生物反应。本研究的目的是比较L929小鼠成纤维细胞对几种玻璃离子水门汀（GIC）的反应，即传统的GIC，树脂改性玻璃离子水门汀（RMGIC）和聚酸改性树脂复合物（PMRC），使用三种不同的方法。1)3-（4，5-二甲基噻唑-2-基）-2，5-二苯基溴化四氮唑（MTT）法2）琼脂扩散试验，3）扫描电镜MTT法显示，L929成纤维细胞在多酸改性树脂复合充填材料上的附着在第1天时过多，第3天时减少（P < 0.05）。各充填材料在第1、3天的细胞增殖率与对照组（100%）比较，差异有统计学意义（P < 0.05）。尽管根据琼脂扩散试验结果，树脂改性玻璃离子水门汀被确定为具有轻微细胞毒性，但组间差异不显著（P > 0.05）。除了我们的体外研究结果外，化学表面分析技术，元素释放的测量，物理表面表征以及微观结构和孔隙率的分析可以更好地理解对填充材料的生物反应。
The choice of filling material is an important factor in the clinical success of root coverage. Therefore, the cytotoxicity of filling materials must be investigated to ensure a safe biological response. The aim of this study was to compare the response of L929 mouse fibroblasts to several glass ionomer cements (GICs), i.e. conventional GIC, resin-modified glass ionomer cement (RMGIC) and polyacid-modified resin composite (PMRC), using three different methods. 1) 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay 2) agar diffusion test, 3) scanning electron microscopyThe MTT test demonstrated that L929 fibroblast attachment to polyacid-modified resin composite filling material was excessive on day I, but decreased on day 3 (P < 0.05). When the cell proliferation percentages of all filling materials were compared with those of the control group (100%) on days 1 and 3, it was observed that statistically significant differences existed (P < 0.05). Although resin-modified glass ionomer cement was determined to be slightly cytotoxic according to the results of agar diffusion tests, differences between the groups were not significant (P > 0.05).In addition to our in vitro research results, chemical surface analysis techniques, measurement of the release of elements, physical surface characterization and analysis of microstructure and porosity can provide a better understanding of the biological response to filling materials.