Characterization in vitro and in vivo of the oxygen dependence of an enzyme/polymer biosensor for monitoring brain glucose
Characterization in vitro and in vivo of the oxygen dependence of an enzyme/polymer biosensor for monitoring brain glucose
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DOI：
10.1016/s0165-0270(02)00170-x
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发表时间：
2002-09-30
影响因子：
3
通讯作者：
O'Neill, RD
中科院分区：
文献类型：
--
作者：
Dixon, BM;Lowry, JP;O'Neill, RD
第一代电流型生物传感器的氧依赖性进行了研究，在体外和体内通过监测其葡萄糖响应作为溶液PO 2的函数。该生物传感器是葡萄糖氧化酶（GOx）修饰的聚（邻苯二胺）涂层铂圆柱电极（Pt/PPD/GOx），已被设计用于在体内的神经化学分析。两种类型的氧探头：一个自我校准的商业宏电极在体外;和碳糊微电极在体内。体外校准表明，对于脑ECF中发现的葡萄糖浓度（类似于0.5 mM）和氧气浓度（类似于50 μ M），Pt/PPD/GOx电极操作中的氧气干扰最小。这一观察结果得到了证实，在清醒的大鼠脑葡萄糖和氧的同时监测体内。然而，在外周组织中正常发现的葡萄糖水平（类似于5 mM）下，氧依赖性是严重的。我们得出结论，Pt/PPD/GOx生物传感器的氧敏感性并不排除其在含有低葡萄糖水平的介质中的可靠使用，如脑ECF。(C)2002 Elsevier Science B. V.保留所有权利。
The oxygen dependence of a first generation amperometric biosensor was investigated in vitro and in vivo by monitoring its glucose response as a function of solution PO2. The biosensor was a glucose oxidase (GOx) modified poly(o-phenylenediamine) coated Pt cylinder electrode (Pt/PPD/GOx) that has been designed for neurochemical analysis in vivo. Two types of oxygen probes were used: a self-calibrating commercial macroelectrode in vitro; and a carbon paste microelectrode in vivo. Calibrations in vitro showed that oxygen interference in the operation of Pt/PPD/GOx electrodes was minimal for concentrations of glucose (similar to0.5 mM) and oxygen (similar to50 muM) found in brain ECF. This observation was confirmed by simultaneous monitoring in vivo of brain glucose and oxygen in the awake rat. However, at levels of glucose normally found in peripheral tissues (similar to5 mM), the oxygen dependence was severe. We conclude that the oxygen sensitivity of Pt/PPD/GOx biosensors does not preclude their reliable use in media containing low glucose levels, such as brain ECF. (C) 2002 Elsevier Science B.V. All rights reserved.