Characterisation of Gel-Forming Mucins Produced In Vivo and In Ex Vivo Conjunctival Explant Cultures.
Characterisation of Gel-Forming Mucins Produced In Vivo and In Ex Vivo Conjunctival Explant Cultures.
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DOI：
10.3390/ijms221910528
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发表时间：
2021-09-29
影响因子：
5.6
通讯作者：
Pintelon I
中科院分区：
文献类型：
--
作者：
Van Acker SI;Van den Bogerd B;Van Acker ZP;Vailionytė A;Haagdorens M;Koppen C;Ní Dhubhghaill S;Dartt DA;Pintelon I
眼表健康的一个关键因素包括眼前泪膜中凝胶形成粘蛋白的存在。结膜杯状细胞是一种特殊的上皮细胞，分泌维持泪膜稳定和体内平衡所必需的粘蛋白。它们的功能障碍可能与一系列眼表炎症疾病和慢性损伤有关。为了获得解决粘蛋白缺乏症的新视角和角度，需要准确评估它们在离体结膜培养中的存在和相应的粘蛋白分泌已成为必要。在体外，杯状细胞显示出凝胶形成粘蛋白的产生和分泌明显减少，并伴有去分化或转分化的迹象。然而，在层粘连蛋白处理的CLP-PEG水凝胶上的外植体培养可以支持凝胶形成粘蛋白的产生。总之，我们挑战了目前通过成像分析仅基于一般粘蛋白（MUC）含量来评估培养杯状细胞存在的范式，这表明需要额外的技术来评估杯状细胞的功能。此外，我们用MUC5B和MUC6拓宽了体内杯状细胞的成胶粘蛋白谱，同时在培养的杯状细胞谱中加入了MUC2和MUC6。
One key element to the health of the ocular surface encompasses the presence of gel-forming mucins in the pre-ocular tear film. Conjunctival goblet cells are specialized epithelial cells that secrete mucins necessary for tear film stability and general homeostasis. Their dysfunction can be linked to a range of ocular surface inflammation disorders and chronic injuries. To obtain new perspectives and angles to tackle mucin deficiency, the need for an accurate evaluation of their presence and corresponding mucin secretion in ex vivo conjunctival cultures has become a requisite. In vitro, goblet cells show a significant decrease in the production and secretion of gel-forming mucins, accompanied by signs of dedifferentiation or transdifferentiation. Explant cultures on laminin-treated CLP-PEG hydrogels can, however, support the production of gel-forming mucins. Together, we challenge the current paradigm to evaluate the presence of cultured goblet cells solely based on their general mucin (MUC) content through imaging analyses, showing the need for additional techniques to assess the functionality of goblet cells. In addition, we broadened the gel-forming mucin profile of in vivo goblet cells with MUC5B and MUC6, while MUC2 and MUC6 is added to the profile of cultured goblet cells.
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影响因子：
4.4
作者：
Lambiase, Alessandro;Micera, Alessandra;Bonini, Stefano
通讯作者：
Bonini, Stefano
影响因子：
4.6
作者：
Hodges, Robin R.;Li, Dayu;Dartt, Darlene A.
通讯作者：
Dartt, Darlene A.
DOI：
10.1016/b978-0-12-374279-7.02019-1
发表时间：
2016-01-01
期刊：
ENCYCLOPEDIA OF IMMUNOBIOLOGY, VOL 2: MOLECULAR IMMUNOLOGY
影响因子：
--
作者：
Johansson, Malin E. V.;Hansson, Gunnar C.
通讯作者：
Hansson, Gunnar C.
影响因子：
4.4
作者：
Agnifili, Luca;Fasanella, Vincenzo;Marchini, Giorgio
通讯作者：
Marchini, Giorgio
影响因子：
4.3
作者：
Haagdorens, Michel;Cepla, Vytautas;Tassignon, Marie-Jose
通讯作者：
Tassignon, Marie-Jose