Baicalin attenuates global cerebral ischemia/reperfusion injury in gerbils via anti-oxidative and anti-apoptotic pathways
Baicalin attenuates global cerebral ischemia/reperfusion injury in gerbils via anti-oxidative and anti-apoptotic pathways
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DOI：
10.1016/j.brainresbull.2011.05.002
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发表时间：
2011-07-15
影响因子：
3.8
通讯作者：
Cai, Jiqun
中科院分区：
文献类型：
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作者：
Cao, Yonggang;Mao, Xiaoyuan;Cai, Jiqun
黄芩苷是从黄芩干燥根中提纯得到的一种重要中草药。本研究观察黄芩苷对沙土鼠短暂性全脑缺血再灌注损伤的神经保护作用。沙土鼠脑缺血后立即腹腔注射黄芩苷50、100、200 mg/kg。再灌流7d后，苏木精-伊红(HE)染色观察大鼠海马CA1区锥体细胞损伤情况。此外，我们还检测了超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、非酶清除剂谷胱甘肽(GSH)等抗氧化酶活性，并测定了海马区丙二醛(MDA)的含量，以了解黄芩苷的潜在保护机制。用实时荧光定量RT-PCR和Western印迹法检测脑缺血大鼠海马区脑源性神经营养因子的mRNA和蛋白表达。用实时荧光定量RT-PCR、Western印迹和caspase-3活性检测检测神经元凋亡的证据。组织病理学检查显示，100 mg/kg和200 mg/kg剂量的黄芩苷可明显减轻缺血所致的神经细胞损伤。黄芩苷治疗组大鼠血清丙二醛水平明显降低，超氧化物歧化酶、谷胱甘肽和谷胱甘肽过氧化物酶活性明显升高。进一步的研究表明，实时定量RT-PCR和Western印迹显示，黄芩苷分别在mRNA和蛋白水平上显著促进了BDNF的表达，抑制了caspase-3的表达。此外，caspase-3活性测定还表明，给予黄芩苷可显著抑制缺血沙土鼠海马区caspase-3的表达。这些发现表明，黄芩苷在沙土鼠全脑缺血中的神经保护作用似乎与其抗氧化和抗凋亡特性有关。(C)2011 Elsevier Inc.保留所有权利。
Baicalin is an important medicinal herb purified from the dry roots of Scutellaria baicalensis Georgi. The present study was undertaken to evaluate the neuroprotective effects of baicalin in gerbils subjected to transient global cerebral ischemic-reperfusion injury. Baicalin at doses of 50, 100 and 200 mg/kg was intraperitoneally injected into the gerbils immediately after cerebral ischemia. Seven days after reperfusion, hematoxylin and eosin (HE) staining was performed to analyze hippocampal CA1 pyramidal damage histopathologically. In addition, in order to understand the potential protective mechanism of baicalin, we examined anti-oxidative enzymes, such superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), non-enzymatic scavenger glutathione (GSH) and measured the content of malondialdehyde (MDA) in hippocampus. The mRNA and protein expressions of BDNF were determined in ischemic hippocampus by real-time RT-PCR and Western blot, respectively. Evidence for neuronal apoptosis was detected by real-time RT-PCR, Western blot and caspase-3 activity measurement. Histopathological examination showed that the administration of baicalin by the dose of 100 and 200 mg/kg significantly attenuated ischemia-induced neuronal cell damage. Reduced level of MDA, obviously elevated activities of SOD and GSH as well as GSH-PX were also found in baicalin-treated groups. Further investigation demonstrated that treatment with baicalin remarkably promoted the expression of BDNF and inhibited the expression of caspase-3 at mRNA and protein levels by real-time RT-PCR and Western blot, respectively. Besides, caspase-3 activity assay also elucidated that the administration of baicalin could significantly suppress caspase-3 in ischemic gerbils hippocampus. Theses findings suggest that baicalin's neuroprotection appears to be associated with its anti-oxidative and anti-apoptotic properties in global cerebral ischemia in the gerbils. (C) 2011 Elsevier Inc. All rights reserved.