Protein oxidation in Type 2 diabetic patients on hemodialysis
Protein oxidation in Type 2 diabetic patients on hemodialysis
复制标题
DOI：
10.1016/j.jdiacomp.2004.11.001
复制
发表时间：
2005-05-01
影响因子：
3
通讯作者：
Ozben, T
中科院分区：
文献类型：
--
作者：
Dursun, E;Timur, M;Ozben, T
背景：氧化应激被认为是糖尿病（DM）及其并发症（包括肾病）之间的统一联系。有许多关于糖尿病患者氧化剂产生增加和抗氧化剂水平降低的报道。透析过程会导致氧化应激。氧化应激的增加可能有助于糖尿病患者氧化蛋白损伤的发展。我们的目的是揭示糖尿病和血液透析（HD）对血浆蛋白氧化修饰的影响。研究方法：我们测量了2型糖尿病（DM）和糖尿病血液透析患者（DHD）以及健康对照参与者的活性羰基衍生物（PCO）、蛋白巯基（P-SH）和还原型谷胱甘肽（GSH）水平。结果：与对照组相比，所有患者组的蛋白质羰基（PCO）含量均显著增加。与DM患者相比，透析过程导致DHD患者透析前后PCO水平额外增加。DHD患者血浆P-SH水平较正常人和DM患者明显降低。糖尿病患者和对照组之间的全血GSH水平没有显着差异。DHD患者中的这一比例显著高于DM患者。结论：我们的结论是，PCO水平增加，糖尿病患者，这种增加是更深刻的DHD患者，表明糖尿病和透析有助于增加蛋白质氧化。DHD患者的P-SH水平较低，而DM患者则没有，这表明透析是导致这种下降的原因。我们建议血浆PCO衍生物作为一种新的特异性氧化蛋白损伤的标志物。(c)2005年爱思唯尔公司All rights reserved.
Background: Oxidative stress is considered to be a unifying link between diabetes mellitus (DM) and its complications, including nephropathy. There have been many reports on increased production of oxidants and decreased level of antioxidants in diabetic patients. The dialysis procedure contributes to oxidative stress. An increase in oxidative stress may contribute to the development of oxidative protein damage in diabetic patients. Our aim was to reveal the effects of diabetes and hemodialysis (HD) on oxidative modifications of plasma proteins. Methods: We measured reactive carbonyl derivates (PCO), protein thiol (P-SH), and reduced glutathione (GSH) levels in Type 2 diabetic (DM) and diabetic hemodialysed patients (DHD) and in healthy control participants. Results: Protein carbonyl (PCO) content increased significantly in all patient groups relative to the controls. The dialysis procedure caused an additional increase in PCO levels in DHD patients before and after dialysis compared with the level in DM patients. There was a significant decrease in P-SH levels in DHD patients compared with the level in healthy participants and DM patients. There was no significant difference in the whole blood GSH levels between the DM patients and control participants. It was significantly higher in DHD patients in comparison to the DM patients. Conclusions: We conclude that PCO level increases in DM patients, and this increase is more profound in DHD patients, indicating that both diabetes and dialysis contribute to increased protein oxidation. The low P-SH level in DHD patients, but not in DM patients, suggests that dialysis is responsible for this decrease. We propose plasma PCO derivate as a novel specific marker for oxidative protein damage. (c) 2005 Elsevier Inc. All rights reserved.