13C-13C NOESY:: An attractive alternative for studying large macromolecules
13C-13C NOESY:: An attractive alternative for studying large macromolecules
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DOI：
10.1021/ja0357036
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发表时间：
2004-01-21
影响因子：
15
通讯作者：
Pierattelli, R
中科院分区：
文献类型：
--
作者：
Bertini, I;Felli, IC;Pierattelli, R
13C直接检测提供了一个有价值的替代1H检测，以克服快速弛豫，因为它的磁矩较小。13C −13C NOESY光谱获得的二聚体蛋白质的分子量为32 000和单体类似物。随着分子质量的增加，13 C − 13 C NOESY光谱的质量提高，而标量为基础的实验变得不那么敏感，预测的分子质量的增加。13 C − 13 C NOESY光谱的二聚体获得了不同的混合时间。可以调整混合时间以主要检测单键相关性，或者可以增加混合时间以检测更长距离处的核之间的相关性。有人提出，基于13 C − 13 C偶极的实验为大分子（如多聚体和大分子复合物）的信号检测和分配提供了一个有前途的工具，而基于标量的实验变得不那么有效。
13C direct detection provides a valuable alternative to1H detection to overcome fast relaxation because of its smaller magnetic moment.13C−13C NOESY spectra were acquired for a dimeric protein of molecular mass 32 000 and for a monomeric analogue. With increasing molecular mass, the quality of13C−13C NOESY spectra improves while the scalar-based experiments become less sensitive, as predicted by the increase in the molecular mass.13C−13C NOESY spectra of the dimer were acquired with different mixing times. The mixing time can be tuned to detect mainly one-bond correlations, or it can be increased to also detect correlations between nuclei at longer distances. It is proposed that13C−13C dipolar-based experiments provide a promising tool for signal detection and assignment in large macromolecules, such as multimeric species and macromolecular complexes, for which scalar-based experiments become less effective.