Amplification efficiency of thermostable DNA polymerases
Amplification efficiency of thermostable DNA polymerases
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DOI：
10.1016/s0003-2697(03)00465-2
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发表时间：
2003-10-15
影响因子：
2.9
通讯作者：
Hogrefe, HH
中科院分区：
文献类型：
--
作者：
Arezi, B;Xing, WM;Hogrefe, HH
采用实时荧光定量聚合酶链式反应结合SYBR Green I检测方法，比较几种聚合酶链式反应酶的扩增效率。在聚合酶链式反应的指数阶段收集的扩增数据具有高度的重复性，基于效率测量的聚合酶链式反应性能比较比基于终点分析的聚合酶链式反应酶性能比较要准确得多。在相同的条件下，使用长度或GC含量百分比不同的五种不同的扩增子模板来测定DNA聚合酶效率。在放大较短靶点时，基于PFU和Taq的配方显示出类似的效率(
The amplification efficiencies of several polymerase chain reaction (PCR) enzymes were compared using real-time quantitative PCR with SYBR Green I detection. Amplification data collected during the exponential phase of PCR are highly reproducible, and PCR enzyme performance comparisons based upon efficiency measurements are considerably more accurate than those based on endpoint analysis. DNA polymerase efficiencies were determined under identical conditions using five different amplicon templates that varied in length or percentage GC content. Pfu- and Taq-based formulations showed similar efficiencies when amplifying shorter targets (