Clustering and dynamics of crowded proteins near membranes and their influence on membrane bending
Clustering and dynamics of crowded proteins near membranes and their influence on membrane bending
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DOI：
10.1073/pnas.1910771116
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发表时间：
2019-12-03
影响因子：
11.1
通讯作者：
Feig, Michael
中科院分区：
文献类型：
--
作者：
Nawrocki, Grzegorz;Im, Wonpil;Feig, Michael
原子分子动力学模拟的浓缩蛋白质溶液中存在的磷脂双层的动态和相互作用在胞质溶胶-膜界面的见解。主要发现是，不知道特异性与膜相互作用的蛋白质优先从膜中排除，在膜表面附近留下耗尽区。因此，有效的蛋白质浓度增加，导致蛋白质接触和聚集增加，而偶尔进入耗尽区的蛋白质在膜附近的蛋白质扩散变得更快。由于蛋白质膜接触是罕见的，在这项研究中，短暂的，脂质双层的结构仍然在很大程度上不受拥挤的蛋白质溶液，但当蛋白质接触脂质头组，小，但统计学上显着的局部膜曲率诱导，平均。
Atomistic molecular dynamics simulations of concentrated protein solutions in the presence of a phospholipid bilayer are presented to gain insights into the dynamics and interactions at the cytosol-membrane interface. The main finding is that proteins that are not known to specifically interact with membranes are preferentially excluded from the membrane, leaving a depletion zone near the membrane surface. As a consequence, effective protein concentrations increase, leading to increased protein contacts and clustering, whereas protein diffusion becomes faster near the membrane for proteins that do occasionally enter the depletion zone. Since protein-membrane contacts are infrequent and short-lived in this study, the structure of the lipid bilayer remains largely unaffected by the crowded protein solution, but when proteins do contact lipid head groups, small but statistically significant local membrane curvature is induced, on average.