Quantification of methanogen cell density in anaerobic granular sludge consortia by fluorescence in-situ hybridization
Quantification of methanogen cell density in anaerobic granular sludge consortia by fluorescence in-situ hybridization
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DOI：
10.2166/wst.2000.0361
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发表时间：
2000-08
影响因子：
2.7
通讯作者：
T. Tagawa;K. Syutsubo;Y. Sekiguchil;A. Ohashi;H. Harada
中科院分区：
文献类型：
--
作者：
T. Tagawa;K. Syutsubo;Y. Sekiguchil;A. Ohashi;H. Harada
采用16SrRNA靶向寡核苷酸全细胞荧光原位杂交技术（Whole cell fluorescence in situ hybridization，FISH）研究了UASB颗粒污泥的微生物生态结构。FISH分析表明，在不同的颗粒污泥源中，结构域的成员占总细胞的28%至53%，而Methanosaeta和Methanobacteriaceae细胞分别占13%至38%和4%至27%。Methanosaeta细胞密度和Methanobacteriaceae细胞密度分别与利用乙酸的甲烷生产活性和利用氢的甲烷生产活性密切相关。
Whole cell fluorescence in-situ hybridization (FISH) with 16S rRNA targeted oligonucleotides was applied to reveal the microbial ecological structure of UASB-grown granular sludge. The FISH analysis indicated that the members of the domain Archaea accounted for 28 to 53% of the total cells in various granular sludge sources, while Methanosaeta and Methanobacteriaceae cells accounted for 13 to 38%, and 4 to 27%, respectively. Methanosaeta cell density and Methanobacteriaceae cell density were strongly correlated, respectively, with acetate-utilizing methane production activity and with hydrogen-utilizing methane production activity.