Chronic maternal and fetal Porphyromonas gingivalis exposure during pregnancy in rabbits
Chronic maternal and fetal Porphyromonas gingivalis exposure during pregnancy in rabbits
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DOI：
10.1016/j.ajog.2004.09.001
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发表时间：
2005-02-01
影响因子：
9.8
通讯作者：
Offenbacher, S
中科院分区：
文献类型：
--
作者：
Boggess, KA;Madianos, PN;Offenbacher, S
目的：本研究是进行开发一个兔模型的母体暴露于牙龈卟啉单胞菌，并确定是否胎儿或胎盘exposedoccurs.Study设计：皮下钢腔植入在8个新西兰白色雌性兔。在妊娠第7天，4只家兔通过小室接种5 × 10（8）CFU/mL活牙龈卟啉单胞菌，4只家兔接种肉汤（对照），并在足月时处死。应用聚合酶链反应检测母胎肝脏和胎盘组织中牙龈卟啉单胞菌。Fisher精确检验被用来比较牙龈卟啉单胞菌检测groups.Results：在暴露剂量，牙龈卟啉单胞菌被检测到33%的母体肝脏，49%的胎盘，和34%的胎儿肝脏相比，没有从controls.Conclusion：慢性母体暴露牙龈卟啉单胞菌的结果全身传播，经胎盘通过，和胎儿暴露。该模型可能有助于研究这种口腔病原体对胎盘和胎儿的影响，并研究微生物在胎盘中的传播。(C)2005年爱思唯尔公司All rights reserved.
Objective: This study was undertaken to develop a rabbit model of maternal exposure to Porphyromonas gingivalis and determine whether fetal or placental exposure occurs.Study design: Subcutaneous steel chambers were implanted in 8 New Zealand White female rabbits. On day 7 of pregnancy, 4 rabbits were inoculated through the chamber with 5 X 10(8) CFU/mL live P gingivalis, and 4 rabbits with broth (controls) and sacrificed at term. Polymerase chain reaction was used to detect P gingivalis in maternal and fetal liver and placenta. Fisher exact test was used to compare P gingivalis detection between groups.Results: Among exposed does, P gingivalis was detected in 33% of the maternal livers, 49% of placentas, and 34% fetal livers compared with none from controls (P < .001).Conclusion: Chronic maternal exposure to P gingivalis results in systemic dissemination, transplacental passage, and fetal exposure. This model may be useful to study placental and fetal effects of this oral pathogen and to study microbial dissemination across the placenta. (C) 2005 Elsevier Inc. All rights reserved.