PREDOMINANCE OF MEMORY T-CELLS (CD4+, CDW29+) OVER NAIVE T-CELLS (CD4+, CD45R+) IN BOTH NORMAL AND DISEASED HUMAN-SKIN
PREDOMINANCE OF MEMORY T-CELLS (CD4+, CDW29+) OVER NAIVE T-CELLS (CD4+, CD45R+) IN BOTH NORMAL AND DISEASED HUMAN-SKIN
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DOI：
10.1007/bf00424268
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发表时间：
1989-01-01
影响因子：
3
通讯作者：
KAPSENBERG, ML
中科院分区：
文献类型：
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作者：
BOS, JD;HAGENAARS, C;KAPSENBERG, ML
测定并统计评估银屑病、特应性皮炎、钱币状皮炎、玫瑰糠疹和扁平苔藓皮损皮肤中 CD3 + T 淋巴细胞及其亚群的绝对数量。将皮肤切片分为水平层，并对 CD3 + T 细胞以及 CD4 + 诱导剂和 CD8 + 抑制细胞毒性 T 细胞亚群的数量进行计数。此外，还评估了诱导T细胞的两个亚群，即“记忆”（4B4+2H4-）和“幼稚”（4B4-2H4+）的绝对数量。出乎意料的是，T细胞的表皮浸润在牛皮癣中最高，在特应性皮炎中最低。在大多数情况下，这种胞吐作用由 CD8 + 抑制性/细胞毒性 T 淋巴细胞主导，玫瑰糠疹中表皮平均 CD4/平均 CD8 比率最小为 0.04，牛皮癣中最大为 0.48。表皮内的诱导 T 细胞几乎完全是 4B4 + 2H4-“记忆”T 细胞亚群，而 4B4-2H4 +“幼稚”T 细胞在病变表皮中极为罕见。在所有研究的疾病中，真皮 T 细胞都获得了类似的结果，即 4B4-2H4 +“初始”T 细胞相对较少。扁平苔藓中 T 细胞对乳头状真皮的浸润最高，其中平均 CD4/平均 CD8 比率为 1.10，是本比较研究中的最小值。特应性皮炎中乳头状浸润的平均 CD4/平均 CD8 比率最高 (4.12)。我们的结果表明，在所研究的慢性炎症性皮肤病中，T 淋巴细胞亚群具有疾病特异性且显着不同的浸润模式。在所有研究的疾病以及正常人类皮肤中（之前报道过），CD4+2H4-CD4+T细胞“记忆”亚群的主要存在似乎表明皮肤免疫系统在诱导T细胞亚群的增加方面是相当单向的。因此，“记忆”亚群的这种优势表明，正常和患病人类皮肤的大多数 T 细胞已经准备好，即已经在 MHC II 环境中遇到了它们的特定配体。
Absolute numbers of CD3 + T lymphocytes and their subpopulations were determined and statistically evaluated in the lesional skin of psoriasis, atopic dermatitis, nummular dermatitis, pityriasis rosea, and lichen planus. Skin sections were divided into horizontal layers and the numbers of CD3 + T cells as well as CD4 + inducer and CD8 + suppressor-cytotoxic T-cell subsets were counted. In addition, absolute numbers of the two subpopulations of inducer T cells, i.e., "memory" (4B4 + 2H4-) and "naive" (4B4- 2H4 +) were evaluated. Unexpectedly, epidermal infiltration by T cells was highest in psoriasis and lowest in atopic dermatitis. In most cases, this exocytosis was dominated by CD8 + suppressor/cytotoxic T lymphocytes, with a minimal epidermal mean CD4/mean CD8 ratio of 0.04 in pityriasis rosea and a maximum of 0.48 in psoriasis. Inducer T cells within the epidermis were almost exclusively of the 4B4 + 2H4- "memory" T-cell subpopulation, whereas 4B4- 2H4 + "naive" T cells were extremely uncommon in lesional epidermis. Similar results were obtained for dermal T cells in all diseases studied, i.e., 4B4- 2H4 + "naive" T cells were relatively rare. Papillary dermis infiltration by T cells was highest in lichen planus where a mean CD4/mean CD8 ratio of 1.10, the minimum in this comparative study, was obtained. The mean CD4/mean CD8 ratio of the papillary infiltrate was highest in atopic dermatitis (4.12). Our results indicate disease-specific and significantly different infiltration patterns of T-lymphocyte subsets in the chronic inflammatory dermatoses investigated. The predominant presence of the CD4 + 2H4- "memory" subpopulation of CD4 + T cells in all diseases studied as well as in normal human skin (reported previously) seems to indicate that the skin immune system is rather unidirectional in its increase in this subpopulation of the inducer T-cell subset. This predominance of the "memory" subpopulation thus indicates that most T cells of normal and diseased human skin are already primed, i.e., have already met their specific ligand in a MHC II context.