Enzyme immunoassay detection of antibodies in canine blastomycosis using Blastomyces dermatitidis lysate antigens.
Enzyme immunoassay detection of antibodies in canine blastomycosis using Blastomyces dermatitidis lysate antigens.
复制标题
使用皮炎芽生菌裂解物抗原对犬芽生菌病中的抗体进行酶免疫分析检测。
DOI：
10.1111/myc.1990.33.9-10.483
复制
发表时间：
1990
期刊：
影响因子：
4.9
通讯作者：
Scalarone,GM
中科院分区：
文献类型：
--
作者：
Seawell,BW;Legendre,AM;Scalarone,GM
本实验室制备的皮炎ABlastomyces dermatitis yeast - lysate抗原（T - 58）用于酶联免疫吸附试验（ELISA），用于检测201份胚菌病犬血清标本中的抗体。此外，还分析了来自芽孢菌病流行地区的36只未感染犬的血清和来自芽孢菌病非流行地区的36只未感染犬的血清。对于确诊为芽孢菌病的犬标本，敏感性值从96.2%（53份治疗前血清）到88.5%（148份治疗后血清）不等。未感染犬的血清反应性极低。疫区血清的阳性反应率为8.3%，非疫区血清的阳性反应率为5.6%。预处理血清的平均ELISA指数为7.66，疫区血清和非疫区标本的平均ELISA指数分别为1.11和1.03。
ABlastomyces dermatitidis yeastphase lysate antigen (T‐58) prepared in our laboratory was used in an enzyme‐ linked immunosorbent assay (ELISA) for the detection of antibodies in 201 serum specimens from dogs with blastomycosis. In addition 36 sera from non‐infected dogs from an endemic area for blastomycosis and 36 sera from non‐infected dogs residing in a blastomycosis non‐endemic area were assayed . Sensitivity values ranged from 96.2 % (53 pre‐treatment sera) to 88.5 % (148 post‐treatment sera) with the specimens from dogs with confirmed blastomycosis. Minimal reactivity was experienced with the sera from non‐infected dogs. Positive reactions were obtained with 8.3 % of the endemic area sera and with 5.6 % of the sera from the non‐endemic area. Mean ELISA index values ranged from 7.66 with the pretreatment sera to 1.11 and 1.03 with the endemic area sera and non‐endemic area specimens respectively.