Involvement of arginine-vasopressin in the diuretic and hypotensive effects of Pereskia grandifolia Haw. (Cactaceae)
Involvement of arginine-vasopressin in the diuretic and hypotensive effects of Pereskia grandifolia Haw. (Cactaceae)
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DOI：
10.1016/j.jep.2012.08.034
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发表时间：
2012-10-31
影响因子：
5.4
通讯作者：
Laverde Junior, Antonio
中科院分区：
文献类型：
--
作者：
Kazama, Caroline Calixto;Uchida, Denise Thiemi;Laverde Junior, Antonio
民族药理学相关性：Pereskia grandifolia Haw.（仙人掌科），俗称“ora-pro-nobis”，在巴西传统医学中被公认为利尿剂，尽管没有科学数据支持这种效果。本工作的目的是评估输液（INFPG）和Pereskia grandifolia.Materials的乙醇提取物（HEPG）的利尿和消肿活性和可能的作用机制和方法：输液（2.5-10%）和HEPG（3-100 mg/kg）经口给药在单剂量或每日（7天）大鼠。测定动物尿液排泄率、pH值、密度、电导率及Na+、K+、Cl-、HCO 3-含量。在收集的血清样本中，电解质，尿素，肌酐，醛固酮，血管加压素和血管紧张素转换酶（ACE）活性的浓度进行了评价。结果：HEPG在急性期和延长期均能显著增加大鼠水排出率，降低尿K ~+和Cl ~-排出量。口服HEPG（30 mg/kg）可显著降低血压正常大鼠的血清加压素水平和平均动脉压（MAP）。所有其他评估参数没有受到任何treatment.Conclusion：结果表明，HEPG可以目前的化合物（S）负责aquaretic活动，没有任何迹象的毒性，这种效果可能涉及减少在阿替尼加压素释放。(C)2012爱思唯尔爱尔兰有限公司保留所有权利。
Ethnopharmacological relevance: Pereskia grandifolia Haw. (Cactaceae), popularly known as "ora-pro-nobis" is well recognized in Brazilian traditional medicine as a diuretic agent, although no scientific data have been published to support this effect. The aim of this work is to evaluate the diuretic and hypotensive activities of the infusion (INFPG) and the ethanol extract (HEPG) of Pereskia grandifolia and possible mechanism of action.Materials and methods: The infusions (2.5-10%) and the HEPG (3-100 mg/kg) were orally administered in a single dose or daily (for seven days) to rats. The urine excretion rate, pH, density, conductivity and content of Na+, K+, Cl- and HCO3- were measured in the urine of saline-loaded animals. In collected serum samples the concentration of electrolytes, urea, creatinine, aldosterone, vasopressin and angiotensin converting enzyme (ACE) activity were evaluated. The involvement of V-2 vasopressin receptor in the diuretic activity and the hypotensive effect of HEPG were also determined.Results: Water excretion rate was significantly increased by HEPG, while the urinary K+ and Cl- excretion was significantly reduced in acute and prolonged treatment. The oral administration of the HEPG (30 mg/kg) significantly reduced serum levels of vasopressin and the mean arterial pressure (MAP) in normotensive rats. All other evaluated parameters have not been affected by any treatment.Conclusion: The results showed that HEPG could present compound(s) responsible for aquaretic activities with no signs of toxicity, and this effect could involve a reduction in the argininevasopressin release. (C) 2012 Elsevier Ireland Ltd. All rights reserved.