Multiple transcripts regulate glucose-triggered mRNA decay of the lactate transporter JEN1 from Saccharomyces cerevisiae
Multiple transcripts regulate glucose-triggered mRNA decay of the lactate transporter JEN1 from Saccharomyces cerevisiae
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DOI：
10.1016/j.bbrc.2005.04.119
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发表时间：
2005-06-24
影响因子：
3.1
通讯作者：
Casal, M
中科院分区：
文献类型：
--
作者：
Andrade, RP;Kötter, P;Casal, M
编码乳酸转运蛋白的酿酒酵母JEN 1基因在转录和转录后水平上都受到强烈的分解代谢抑制。在向诱导的细胞培养物中加入葡萄糖、果糖或甘露糖脉冲后，JEN 1 mRNA衰减大大加速。JEN 1转录物的5 'UTR和3' UTR的定位揭示了位于位置-51、+391或+972的多个转录起始位点，这取决于细胞培养条件。JEN 1（+391）转录本的存在与葡萄糖触发的JEN 1（-51）转录本的快速mRNA降解相关，而当小转录本从位置+972开始时，JEN 1（-51）mRNA周转率不受影响。在所有测试条件下，过表达的JEN 1（+391）转录物加速JEN 1（-51）mRNA的衰减，但不翻译。我们认为JEN 1（+391）转录本可能具有“传感器样”功能，调节葡萄糖触发的JEN 1（-51）蛋白编码mRNA的降解。(c)2005年爱思唯尔公司All rights reserved.
The Saccharomyces cerevisiae JEN1 gene encoding the lactate transporter undergoes strong catabolic repression at both transcriptional and post-transcriptional levels. JEN1 mRNA decay is greatly accelerated upon the addition of a pulse of glucose, fructose or mannose to induced Cell Cultures. Mapping of the 5'UTRs and 3'UTRs of JEN1 transcripts revealed multiple transcription start-sites located at position -51, +391 or +972, depending on the Cell Culture conditions. The presence of the JEN1(+391) transcript correlated with rapid glucose-triggered mRNA degradation of the JEN1(-51) transcript, whereas when the small transcript started at position +972, the JEN1(-51) mRNA turnover rate was unaffected. Overexpressed JEN1(+391) transcript accelerated JEN1(-51) mRNA decay in all conditions tested but was not translated. We propose that the JEN1(+391) transcript may have a "sensor-like" function, regulating glucose-triggered degradation of JEN1(-51) protein-coding mRNA. (c) 2005 Elsevier Inc. All rights reserved.