SIMPLE DETERMINATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 SYNCYTIUM-INDUCING V3 GENOTYPE BY PCR
SIMPLE DETERMINATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 SYNCYTIUM-INDUCING V3 GENOTYPE BY PCR
复制标题
DOI：
10.1128/jcm.33.4.906-911.1995
复制
发表时间：
1995-04-01
影响因子：
9.4
通讯作者：
SCHUITEMAKER, H
中科院分区：
文献类型：
--
作者：
FOUCHIER, RAM;BROUWER, M;SCHUITEMAKER, H
人类免疫缺陷病毒1型(HIV-1)表型变异在艾滋病的发病机制中起着重要作用。合胞体诱导(SI)HIV-1分离株的存在；感染者与CD4(+)T细胞的迅速下降、疾病的快速进展以及艾滋病确诊后生存时间的缩短有关。在402株HIV-1原代分离株中，97%的人证实了HIV-1的SI能力与gp120基因第三可变结构域(V3)上306和/或320位带正电荷的氨基酸残基的存在密切相关，表明V3基因可能有助于预测病毒的表型。V3DNA序列显示，与病毒表型有关的氨基酸残基的密码子使用非常有限。基于这种有限的SI特异性DNA序列变异，设计了4个SI特异性寡核苷酸，用于选择性扩增SI而不是非SI HIV-1分离株的V3。这种聚合酶链式反应分析可以根据V3基因预测HIV-1分离株的生物学表型，并可能被证明是监测HIV-1分离株在感染个体中的SI能力的有用方法。
Human immunodeficiency virus type 1 (HIV-1) phenotype variability plays an important role in the pathogenesis of AIDS. The presence of syncytium-inducing (SI) HIV-1 isolates in; infected individuals is associated with a rapid decline of CD4(+) T cells, rapid disease progression, and reduced survival time after AIDS diagnosis. The strong association between the SI capacity of HIV-1 and the presence of positively charged amino acid residues at positions 306 and/or 320 in the third variable domain (V3) of gp120 could here be confirmed in 97% of 402 primary HIV-1 isolates, indicating that the V3 genotype may be useful for prediction of the viral phenotype. The V3 DNA sequences revealed a remarkably limited codon usage for the amino acid residues that are responsible for virus phenotype. On the basis of this limited SI-specific DNA sequence variation, four SI-specific oligonucleotides were designed for selective amplification of V3 from SI but not non-SI HIV-1 isolates. This PCR analysis allowed the prediction of the biological phenotype of HIV-1 isolates on the basis of the V3 genotype and may prove to be useful for monitoring SI capacity of HIV-1 isolates in infected individuals.