Expression of manganese superoxide dismutase in esophageal and gastric cancers
Expression of manganese superoxide dismutase in esophageal and gastric cancers
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DOI：
10.1007/s005350050181
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发表时间：
1998-12-01
影响因子：
6.3
通讯作者：
Ohyanagi, H
中科院分区：
文献类型：
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作者：
Izutani, R;Asano, S;Ohyanagi, H
癌症放化疗的杀伤活性与活性氧的产生密切相关，而肿瘤本身产生的活性氧清除剂与治疗耐药的关系越来越受到人们的关注。人们认为，锰超氧化物歧化酶（MnSOD）与其他抗氧化酶协同作用，可以保护宿主细胞免受氧化应激。在这项研究中，我们使用定量聚合酶链反应测定来测量食管癌和胃癌患者切除标本中的 MnSOD mRNA。在食管癌和胃癌中，癌组织中 MnSOD mRNA 水平均显着高于非癌组织（P < 0.01）。胃癌组织中MnSOD mRNA水平显着高于食管癌组织（P < 0.01）。通过使用酶联免疫吸附测定法测量切除标本中的 MnSOD 含量，并通过免疫组织化学方法检查其位置，进一步研究了 MnSOD 在癌组织中的重要性。癌组织中 MnSOD 的上调很可能是针对抗癌疗法的一种保护机制，已知抗癌疗法会产生超氧自由基，这是其消除谣言活性的关键组成部分。
The tumor-killing activity of radiotherapy and chemotherapy for cancer is closely associated with the production of active oxygen, and the relation between therapeutic resistance and active oxygen scavengers produced by the tumor itself is gaining more attention. It is considered that manganese superoxide dismutase (MnSOD) protects host cells from oxidative stress, in synergy with other antioxidant enzymes. In this study, we used a quantitative polymerase chain reaction assay to measure MnSOD mRNA in resected specimens from patients with esophageal and gastric cancers. In both esophageal and gastric cancers, the level of MnSOD mRNA was significantly elevated in cancer tissue compared to non-cancer tissue (P < 0.01). In gastric cancer tissue, the MnSOD mRNA level was significantly higher than in esophageal cancer tissue (P < 0.01). The significance of MnSOD in cancer tissue was investigated further by measuring MnSOD content in resected specimens using an enzyme-linked immunosorbent assay, and by examining its location by an immunohistochemical method. Upregulation of MnSOD in cancer tissue most likely serves as a protective mechanism against anti-cancer therapies known to produce superoxide radicals as a key component of their rumor-killing activity.