Characteristics and toxicity assessment of electrospun gelatin/PCL nanofibrous scaffold loaded with graphene in vitro and in vivo
Characteristics and toxicity assessment of electrospun gelatin/PCL nanofibrous scaffold loaded with graphene in vitro and in vivo
复制标题
负载石墨烯的电纺明胶/PCL纳米纤维支架的体内外特性及毒性评估
DOI：
10.2147/ijn.s204971
复制
发表时间：
2019-01-01
影响因子：
8
通讯作者：
Li, Fen
中科院分区：
文献类型：
--
作者：
Chen, Xi;Feng, Bei;Li, Fen
背景资料：石墨烯负载的静电纺丝明胶/聚己内酯（Gt/PCL）纳米纤维支架是一种新型纳米材料，具有独特的强导电性，在心血管组织工程中具有广泛的应用前景，包括房室传导阻滞的旁路通道。目的：成功制备了静电纺丝Gt/PCL/石墨烯纳米纤维毡。扫描电子显微镜显示，具有石墨烯的纤维是光滑和均匀的。在体外，为了确定支架的生物相容性，用新生大鼠心室肌细胞接种具有不同分数的石墨烯的混合支架。在体内，将含有不同浓度石墨烯的Gt/PCL支架植入大鼠体内4、8和12周。结果如下：CCK-8测定和组织病理学染色（包括DAPI、cTNT和CX43）表明，如果石墨烯的质量分数低于0.5%，则细胞在杂化支架上生长和存活良好。植入大鼠体内4、8、12周后，HE染色结果显示纳米材料周围无炎性细胞聚集。结论：结果表明，负载石墨烯的Gt/PCL纳米纤维支架具有良好的导电性和生物学性能，可作为治疗房室传导阻滞的合适支架材料。这些发现缓解了安全性问题，并为石墨烯负载的Gt/PCL的潜在应用提供了新的见解，为全面的体内研究提供了坚实的基础。
Background: Electrospun gelatin/polycaprolactone (Gt/PCL) nanofibrous scaffolds loaded with graphene are novel nanomaterials with the uniquely strong property of electrical conductivity, which have been widely investigated for their potential applications in cardiovascular tissue engineering, including in bypass tracts for atrioventricular block. Purpose: Electrospun Gt/PCL/graphene nanofibrous mats were successfully produced. Scanning electron micrography showed that the fibers with graphene were smooth and homogeneous. In vitro, to determine the biocompatibility of the scaffolds, hybrid scaffolds with different fractions of graphene were seeded with neonatal rat ventricular myocytes. In vivo, Gt/PCL scaffolds with different concentrations of graphene were implanted into rats for 4, 8 and 12 weeks. Results: CCK-8 assays and histopathological staining (including DAPI, cTNT, and CX43) indicated that cells grew and survived well on the hybrid scaffolds if the mass fraction of graphene was lower than 0.5%. After implanting into rats for 4, 8 or 12 weeks, there was no gathering of inflammatory cells around the nanomaterials according to the HE staining results. Conclusion: The results indicate that Gt/PCL nanofibrous scaffolds loaded with graphene have favorable electrical conductivity and biological properties and may be suitable scaffolds for use in the treatment of atrioventricular block. These findings alleviate safety concerns and provide novel insights into the potential applications of Gt/PCL loaded with graphene, offering a solid foundation for comprehensive in vivo studies.