Unidimensional thin-layer chromatography of phospholipids on boric acid-impregnated plates.
Unidimensional thin-layer chromatography of phospholipids on boric acid-impregnated plates.
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发表时间：
1982-05
影响因子：
6.5
通讯作者：
J. Fine;H. Sprecher
中科院分区：
文献类型：
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作者：
J. Fine;H. Sprecher
已经开发出用于分离磷脂的一维薄层色谱系统。该方法采用浸渍硼酸的预涂板，能够解析八种磷脂标准品，包括磷脂酰肌醇、磷脂酰丝氨酸和磷脂酰甘油。使用这种方法将大鼠的肝脏、肾脏和血小板磷脂分为六大类。通过二维薄层色谱法判断每种物质都是纯的。使用硼酸薄层色谱分离磷脂应该得到广泛的应用。
A unidimensional thin-layer chromatography system for the separation of phospholipids has been developed. The method employs precoated plates impregnated with boric acid and is capable of resolving eight phospholipid standards, including phosphatidylinositol, phosphatidylserine, and phosphatidylglycerol. Liver, kidney, and platelet phospholipids of the rat were resolved into six major classes using this method. Each was judged to be pure by two-dimensional thin-layer chromatography. The use of boric acid thin-layer chromatography for the separation of phospholipids should find wide application.